ArticleNucleus (Austin, Tex.)2016
Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli.
Article in Nucleus (Austin, Tex.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Nuclear envelope-dependent heterochromatin positioning gates the timing of nucleolar assembly in the early embryo.bioRxiv : the preprint server for biology · 2026Article
- Mechanobiology of the Nucleolus.Biology of the cell · 2026Review
- SUN5 interacts with TRIM28, enhancing IκBα ubiquitination to promote glycolysis in colorectal cancer cells.Acta biochimica et biophysica Sinica · 2025Article
- Depletion of SUN1/2 induces heterochromatin accrual in mesenchymal stem cells during adipogenesis.Communications biology · 2025Article
- LINC complex alterations are a key feature of sporadic and familial ALS/FTD.Acta neuropathologica communications · 2024Article
- SUN1/2 controls macrophage polarization via modulating nuclear size and stiffness.Nature communications · 2023Article
- ATXN3 controls DNA replication and transcription by regulating chromatin structure.Nucleic acids research · 2023Article
- Methylated histones on mitotic chromosomes promote topoisomerase IIα function for high fidelity chromosome segregation.iScience · 2023Article
- Review
- From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues.Biomolecules · 2021Review
- The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal.Scientific reports · 2021Article
- Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention.Journal of experimental & clinical cancer research : CR · 2021Article
- Non-Canonical Functions of the Gamma-Tubulin Meshwork in the Regulation of the Nuclear Architecture.Cancers · 2020Review
- Genome communication in plants mediated by organelle-n-ucleus-located proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Computational analysis of morphological and molecular features in gastric cancer tissues.Cancer medicine · 2020Article
- A cohesin/HUSH- and LINC-dependent pathway controls ribosomal DNA double-strand break repair.Genes & development · 2019Article
- OPENER Is a Nuclear Envelope and Mitochondria Localized Protein Required for Cell Cycle Progression in Arabidopsis.The Plant cell · 2019Article
- Phase-to-Phase With Nucleoli - Stress Responses, Protein Aggregation and Novel Roles of RNA.Frontiers in cellular neuroscience · 2019Review
- SUN1 Regulates HIV-1 Nuclear Import in a Manner Dependent on the Interaction between the Viral Capsid and Cellular Cyclophilin A.Journal of virology · 2018Article
- Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function.Molecular and cellular biology · 2017Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A supervised machine learning algorithm, which is qualified for image classification and analyzing similarities, is based on multiple discriminative morphological features that are automatically assembled during the learning processes. The algorithm is suitable for population-based analysis of images of biological materials that are generally complex and heterogeneous. Here we used the algorithm wndchrm to quantify the effects on nucleolar morphology of the loss of the components of nuclear envelope in a human mammary epithelial cell line. The linker of nucleoskeleton and cytoskeleton (LINC) complex, an assembly of nuclear envelope proteins comprising mainly members of the SUN and nesprin families, connects the nuclear lamina and cytoskeletal filaments. The components of the LINC complex are markedly deficient in breast cancer tissues. We found that a reduction in the levels of SUN1, SUN2, and lamin A/C led to significant changes in morphologies that were computationally classified using wndchrm with approximately 100% accuracy. In particular, depletion of SUN1 caused nucleolar hypertrophy and reduced rRNA synthesis. Further, wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. Our unbiased morphological quantitation strategies using wndchrm revealed an unexpected link between the components of the LINC complex and the morphologies of nucleoli that serves as an indicator of the malignant phenotype of breast cancer cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.