ArticleMolecular biotechnology2016
Cationic β-Cyclodextrin-Chitosan Conjugates as Potential Carrier for pmCherry-C1 Gene Delivery.
Article in Molecular biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Potential Use of Microbial Surfactant in Microemulsion Drug Delivery System: A Systematic Review.Drug design, development and therapy · 2020Pooled it
- Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines.Diagnostic pathology · 2024Article
- A Predictive and an Optimization Mathematical Model for Device Design in Cardiac Pulsed Field Ablation Using Design of Experiments.Journal of cardiovascular development and disease · 2023Article
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Cytotoxicity Effects of Curcumin Loaded on Chitosan Alginate Nanospheres on the KMBC-10 Spheroids Cell Line.International journal of nanomedicine · 2021Article
- The effective removal of phenol from aqueous solution via adsorption on CS/β-CD/CTA multicomponent adsorbent and its application for COD degradation of drilling wastewater.Environmental science and pollution research international · 2020Article
- Nano-Graphene Oxide-supported APTES-Spermine, as Gene Delivery System, for Transfection of pEGFP-p53 into Breast Cancer Cell Lines.Drug design, development and therapy · 2020Article
- Magnetogel Nanospheres Composed of Cisplatin-Loaded Alginate/B-Cyclodextrin as Controlled Release Drug Delivery.Advanced pharmaceutical bulletin · 2019Article
- Biomacromolecules as carriers in drug delivery and tissue engineering.Acta pharmaceutica Sinica. B · 2018Review
- [Effects of microRNA-140 gene transfection with nucleus localization signal linked nucleic kinase substrate short peptide conjugated chitosan on rabbit articular chondrocytes].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017Article
- Synthesis, Antioxidant, Cytotoxicity, Induce Apoptosis Investigation and Docking Study of New Halogenated Dihydropyrano[3,2-Iranian journal of pharmaceutical research : IJPRArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study cationic β-cyclodextrin-chitosan-mediated nanoparticles were used to transfer pmCherry-C1 into glioblastoma cells and their transfection efficiency were compared to lipofectamine and electroporation. Physicochemical characteristics of nanoparticles were evaluated by photon correlation spectroscopy and scanning electron microscopy. Electrophoretic nuclease resistance and stability assays were used to check the protection of DNA from nucleases digestion. mCherry reporter construct was used for visualization, followed by quantitation of cell survival and gene expression by fluorescence-activated cell sorting analysis and fluorescence microscopy. Particle size was approximately 200 nm and did not change at 4 °C even after 12 weeks. Importantly, the positively charged complexes interacted with DNA could serve as an efficient DNA delivery systems. Most of the gene was associated with the nanoparticles and was efficiently protected from DNAse I digestion. More than 80 % of transfected cells expressed mCherry efficiently.
Indexed as
Identifiers
26961910What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.