ArticleNature communications2016
Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- High-throughput recovery of integron cassettes for gene discovery screens.Nature microbiology · 2026Article
- Article
- Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons.Scientific reports · 2026Article
- Chromosomal integrons are genetically and functionally isolated units of genomes.Nucleic acids research · 2024Article
- Isolation and Genomic Characterization of Schaalia turicensis from a Patient with Gonococcal Urethritis, Thailand.Current microbiology · 2024Article
- Screening Key Sites of Class 2 Integron Integrase that Impact Recombination Efficiency.Current microbiology · 2024Article
- Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems.Science advances · 2024Article
- Article
- Coexistence of tet(A) and blaEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Genomic Study of Chromosomally and Plasmid-Mediated Multidrug Resistance and Virulence Determinants in Klebsiella Pneumoniae Isolates Obtained from a Tertiary Hospital in Al-Kharj, KSA.Antibiotics (Basel, Switzerland) · 2022Article
- Mechanisms of protein evolution.Protein science : a publication of the Protein Society · 2022Review
- Unbridled Integrons: A Matter of Host Factors.Cells · 2022Review
- Article
- Structure-specific DNA recombination sites: Design, validation, and machine learning-based refinement.Science advances · 2020Article
- Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance.Journal of visualized experiments : JoVE · 2018Article
- Class 1 integrons are low-cost structures in Escherichia coli.The ISME journal · 2017Article
- An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli.Microbial cell factories · 2017Article
- Article
- Characterization of Novel Integrons, InFrontiers in microbiology · 2017Article
- Efficiency of integron cassette insertion in correct orientation is ensured by the interplay of the three unpaired features of attC recombination sites.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tyrosine (Y)-recombinases have evolved to deliver mechanistically different reactions on a variety of substrates, but these evolutionary transitions are poorly understood. Among them, integron integrases are hybrid systems recombining single- and double-stranded DNA partners. These reactions are asymmetric and need a replicative resolution pathway, an exception to the canonical second strand exchange model of Y-recombinases. Integron integrases possess a specific domain for this specialized pathway. Here we show that despite this, integrases are still capable of efficiently operating the ancestral second strand exchange in symmetrical reactions between double-stranded substrates. During these reactions, both strands are reactive and Holliday junction resolution can follow either pathway. A novel deep-sequencing approach allows mapping of the crossover point for the second strand exchange. The persistence of the ancestral activity in integrases illustrates their robustness and shows that innovation towards new recombination substrates and resolution pathways was a smooth evolutionary process.
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