Evidence map›Paper›PMID 26960732›Full record

ArticlePlant physiology2016

Gatekeeper Tyrosine Phosphorylation of SYMRK Is Essential for Synchronizing the Epidermal and Cortical Responses in Root Nodule Symbiosis.

Sudip Saha, Anindita Paul, Laura Herring, Ayan Dutta, Avisek Bhattacharya, Sandip Samaddar, Michael B Goshe, Maitrayee DasGupta

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
7.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
  2. Nodule-specificaBIOTECH · 2025
    Article
  3. Article
  4. Review
  5. Molecular Basis of Root Nodule Symbiosis betweenPlants (Basel, Switzerland) · 2020
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Sudip SahaDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).ORCID 0000-0001-9742-3306
Anindita PaulDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).
Laura HerringDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).ORCID 0000-0003-4496-7312
Ayan DuttaDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).
Avisek BhattacharyaDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).
Sandip SamaddarDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).
Michael B GosheDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.).
Maitrayee DasGuptaDepartment of Biochemistry, University of Calcutta, Kolkata 700019, India (S. Saha, A.P., A.D., A.B., S. Samaddar, M.D.); andDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (L.H., M.B.G.) maitrayee_d@hotmail.com.ORCID 0000-0002-7260-3746
University of Calcutta · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Symbiosis receptor kinase (SYMRK) is indispensable for activation of root nodule symbiosis (RNS) at both epidermal and cortical levels and is functionally conserved in legumes. Previously, we reported SYMRK to be phosphorylated on "gatekeeper" Tyr both in vitro as well as in planta. Since gatekeeper phosphorylation was not necessary for activity, the significance remained elusive. Herein, we show that substituting gatekeeper with nonphosphorylatable residues like Phe or Ala significantly affected autophosphorylation on selected targets on activation segment/αEF and β3-αC loop of SYMRK. In addition, the same gatekeeper mutants failed to restore proper symbiotic features in a symrk null mutant where rhizobial invasion of the epidermis and nodule organogenesis was unaffected but rhizobia remain restricted to the epidermis in infection threads migrating parallel to the longitudinal axis of the root, resulting in extensive infection patches at the nodule apex. Thus, gatekeeper phosphorylation is critical for synchronizing epidermal/cortical responses in RNS.

Indexed as

SymbiosisAmino Acid SequenceCarrier ProteinsFabaceaeGene Expression Regulation, PlantGenes, PlantMutagenesisMutationPhenotypePhosphoamino AcidsPhosphorylationPlant EpidermisPlant ProteinsPlant Root NodulationPlant RootsProtein KinasesCarrier ProteinsPhosphoamino AcidsPlant ProteinsProtein KinasesTyrosine

Identifiers

PMID26960732
PMCPMC4854696
OpenAlexW2296262839

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.