ArticleScientific reports2016
Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Application of a derivative of human defensin 5 to treat ionizing radiation-induced enterogenic infection.Journal of radiation research · 2024Article
- Molecular dynamics simulations of human α-defensin 5 (HD5) crossing gram-negative bacterial membrane.PloS one · 2023Article
- Article
- Evaluation of the Binding Mechanism of Human Defensin 5 in a Bacterial Membrane: A Simulation Study.International journal of molecular sciences · 2021Article
- The penetration of human defensin 5 (HD5) through bacterial outer membrane: simulation studies.Journal of molecular modeling · 2021Article
- Structure and antimicrobial activity of NCR169, a nodule-specific cysteine-rich peptide of Medicago truncatula.Scientific reports · 2021Article
- The inhibitory effect of human DEFA5 in growth of gastric cancer by targeting BMI1.Cancer science · 2021Observational
- Disulfides from the Brown AlgaMarine drugs · 2020Article
- Human Intestinal Defensin 5 Inhibits SARS-CoV-2 Invasion by Cloaking ACE2.Gastroenterology · 2020Article
- Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn's disease model mice.Life science alliance · 2020Article
- Article
- Paneth cell α-defensins HD-5 and HD-6 display differential degradation into active antimicrobial fragments.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Myc-induced nuclear antigen constrains a latent intestinal epithelial cell-intrinsic anthelmintic pathway.PloS one · 2019Article
- Evaluation of an Improved Chitosan Scaffold Cross-Linked With Polyvinyl Alcohol and Amine Coupling Through 1-Ethyl-3-(3-Dimethyl Aminopropyl)-Carbodiimide (EDC) and 2 N-Hydroxysuccinimide (NHS) for Corneal Applications.Open access Macedonian journal of medical sciences · 2018Article
- The adsorption of human defensin 5 on bacterial membranes: simulation studies.Journal of molecular modeling · 2018Article
- Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.Chemical reviews · 2018Review
- A Simplified Derivative of Human Defensin 5 with Potent and Efficient Activity against Multidrug-Resistant Acinetobacter baumannii.Antimicrobial agents and chemotherapy · 2018Article
- Antimicrobial Peptides Secreted From Human Cryopreserved Viable Amniotic Membrane Contribute to its Antibacterial Activity.Scientific reports · 2017Article
- Guardians of the Gut: Enteric Defensins.Frontiers in microbiology · 2017Review
- Disulfide cross-linking influences symbiotic activities of nodule peptide NCR247.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidized human defensin 5 (HD5OX), a Paneth cell-secreted antibacterial peptide with three characteristic disulfide bonds, protects the host from invasion by morbigenous microbes in the small intestine. HD5OX can be reduced by thioredoxin (Trx) in vitro, while the biochemical properties of the reduced linear peptide, HD5RED, remain unclear. Here, we first confirm that HD5RED does exist in vivo. Furthermore, we reveal that the recruitment of HD5RED to the outer membrane of Gram-negative bacteria and to the anionic lipid A is lower than that of HD5OX, and HD5RED is less efficient in penetrating bacterial outer and inner membranes and inducing membrane depolarization, which confers an attenuated antibacterial activity to HD5RED. However, due to its higher structural flexibility, the binding of HD5RED to bacterial lipopolysaccharide (LPS) is markedly stronger than that of HD5OX. Consequently, HD5RED is more effective in suppressing the production of the pro-inflammatory cytokine TNF-α in LPS-stimulated macrophages by blocking the interaction between LPS and LPS-binding protein, thus suggesting that HD5RED might act as a scavenger to neutralize LPS in the gut. This study provides insights into the antibacterial and immunoregulatory effects of HD5RED and expands the known repertoire of the enteric defensins.
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