ArticleScientific reports2016
Intracellular acidification is required for full activation of the sweet taste receptor by miraculin.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Effect of Miracle Berry on Taste Modification Properties Among Adults Living in Australia: A Multi-Phase Study Protocol.Food science & nutrition · 2026Article
- Miracle Fruit, a Potential Taste-modifier to Improve Food Preferences: A Review.Current nutrition reports · 2024Review
- Mechanisms and Functions of Sweet Reception in Oral and Extraoral Organs.International journal of molecular sciences · 2024Review
- Genetic determinants of food preferences: a systematic review of observational studies.BMC nutrition · 2024Article
- Substrate binding and inhibition of the anion exchanger 1 transporter.Nature structural & molecular biology · 2023Article
- Prediction of dynamic allostery for the transmembrane domain of the sweet taste receptor subunit, TAS1R3.Communications biology · 2023Article
- Cellular mechanisms of taste disturbance induced by the non-steroidal anti-inflammatory drug, diclofenac, in mice.Frontiers in cellular neuroscience · 2023Article
- Miracle fruit seed as a potential supplement for the treatment of learning and memory disorders in Alzheimer's disease.Frontiers in pharmacology · 2022Article
- Environmental risk assessment of transgenic miraculin-accumulating tomato in a confined field trial in Japan.Plant biotechnology (Tokyo, Japan) · 2021Article
- Safety of dried fruits ofEFSA journal. European Food Safety Authority · 2021Article
- The Chromosome-Level Genome of Miracle Fruit (Frontiers in plant science · 2021Article
- Miracle Berry as a Potential Supplement in the Control of Metabolic Risk Factors in Cancer.Antioxidants (Basel, Switzerland) · 2020Review
- Colorimetric and fluorometric dual-channel ratiometric determination of fungicide cymoxanil based on analyte-induced aggregation of silver nanoparticles and dually emitting carbon dots.Mikrochimica acta · 2019Article
- Allelic Variation in Taste Genes Is Associated with Taste and Diet Preferences and Dental Caries.Nutrients · 2019Article
- Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits.The Journal of biological chemistry · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acidification of the glycoprotein, miraculin (MCL), induces sweet taste in humans, but not in mice. The sweet taste induced by MCL is more intense when acidification occurs with weak acids as opposed to strong acids. MCL interacts with the human sweet receptor subunit hTAS1R2, but the mechanisms by which the acidification of MCL activates the sweet taste receptor remain largely unexplored. The work reported here speaks directly to this activation by utilizing a sweet receptor TAS1R2 + TAS1R3 assay. In accordance with previous data, MCL-applied cells displayed a pH dependence with citric acid (weak acid) being right shifted to that with hydrochloric acid (strong acid). When histidine residues in both the intracellular and extracellular region of hTAS1R2 were exchanged for alanine, taste-modifying effect of MCL was reduced or abolished. Stronger intracellular acidification of HEK293 cells was induced by citric acid than by HCl and taste-modifying effect of MCL was proportional to intracellular pH regardless of types of acids. These results suggest that intracellular acidity is required for full activation of the sweet taste receptor by MCL.
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