ArticleActa crystallographica. Section D, Structural biology2016
Data-collection strategy for challenging native SAD phasing.
Article in Acta crystallographica. Section D, Structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 48 citations in OpenAlex.
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- Application of sulfur SAD to small crystals with a large asymmetric unit and anomalous substructure.Acta crystallographica. Section D, Structural biology · 2022Article
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- Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state.PLoS biology · 2020Article
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- Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases.Acta crystallographica. Section D, Structural biology · 2020Article
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- Making routine native SAD a reality: lessons from beamline X06DA at the Swiss Light Source.Acta crystallographica. Section D, Structural biology · 2019Article
- Structure of the heterophilic interaction between the nectin-like 4 and nectin-like 1 molecules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nucleobase carbonyl groups are poor MgRNA (New York, N.Y.) · 2019Article
- Combinatorial use of disulfide bridges and native sulfur-SAD phasing for rapid structure determination of coiled-coils.Bioscience reports · 2018Article
- Locking loop movement in the ubiquinone pocket of complex I disengages the proton pumps.Nature communications · 2018Article
- Calibration of rotation axes for multi-axis goniometers in macromolecular crystallography.Journal of applied crystallography · 2018Article
- Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.Nature communications · 2018Article
- Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.Journal of visualized experiments : JoVE · 2018Article
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11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent improvements in data-collection strategies have pushed the limits of native SAD (single-wavelength anomalous diffraction) phasing, a method that uses the weak anomalous signal of light elements naturally present in macromolecules. These involve the merging of multiple data sets from either multiple crystals or from a single crystal collected in multiple orientations at a low X-ray dose. Both approaches yield data of high multiplicity while minimizing radiation damage and systematic error, thus ensuring accurate measurements of the anomalous differences. Here, the combined use of these two strategies is described to solve cases of native SAD phasing that were particular challenges: the integral membrane diacylglycerol kinase (DgkA) with a low Bijvoet ratio of 1% and the large 200 kDa complex of the CRISPR-associated endonuclease (Cas9) bound to guide RNA and target DNA crystallized in the low-symmetry space group C2. The optimal native SAD data-collection strategy based on systematic measurements performed on the 266 kDa multiprotein/multiligand tubulin complex is discussed.
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