Evidence map›Paper›PMID 26960126›Full record

ArticleActa crystallographica. Section D, Structural biology2016

Radiation-damage-induced phasing: a case study using UV irradiation with light-emitting diodes.

Daniele de Sanctis, Chloe Zubieta, Franck Felisaz, Hugo Caserotto, Max H Nanao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section D, Structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. X-ray and UV radiation-damage-induced phasing using synchrotron serial crystallography.Acta crystallographica. Section D, Structural biology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daniele de SanctisESRF, The European Synchrotron, 71 Rue des Martyrs, 38000 Grenoble, France.
Chloe ZubietaCNRS, Université Grenoble Alpes, CEA, DSV, INRA, iRTSV, Laboratoire de Physiologie Cellulaire and Végétale, UMR 5168, 38054 Grenoble, France.
Franck FelisazEuropean Molecular Biology Laboratory, 71 avenue des Martyrs, CS 90181, F-38042 Grenoble Cedex 9, France.
Hugo CaserottoESRF, The European Synchrotron, 71 Rue des Martyrs, 38000 Grenoble, France.
Max H NanaoEuropean Molecular Biology Laboratory, 71 avenue des Martyrs, CS 90181, F-38042 Grenoble Cedex 9, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exposure to X-rays, high-intensity visible light or ultraviolet radiation results in alterations to protein structure such as the breakage of disulfide bonds, the loss of electron density at electron-rich centres and the movement of side chains. These specific changes can be exploited in order to obtain phase information. Here, a case study using insulin to illustrate each step of the radiation-damage-induced phasing (RIP) method is presented. Unlike a traditional X-ray-induced damage step, specific damage is introduced via ultraviolet light-emitting diodes (UV-LEDs). In contrast to UV lasers, UV-LEDs have the advantages of small size, low cost and relative ease of use.

Indexed as

CrystallizationCrystallography, X-RayDisulfidesEquipment DesignInsulinModels, MolecularProtein ConformationUltraviolet RaysDisulfidesInsulindisulfide bondslight-emitting diodesphasingradiation damageRIPSIRultraviolet lightUV

Identifiers

PMID26960126
PMCPMC4784670

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.