ArticleElectrophoresis2016
LC-MS/MS of permethylated N-glycans derived from model and human blood serum glycoproteins.
Article in Electrophoresis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Mannose-binding analysis and biological application of pradimicins.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Article
- Toward robust N-glycomics of various tissue samples that may contain glycans with unknown or unexpected structures.Scientific reports · 2021Article
- Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.Journal of the American Chemical Society · 2021Article
- A General New Method for Calculating the Molecular Nonpolar Surface for Analysis of LC-MS Data.International journal of mass spectrometry · 2021Article
- Glycoproteogenomics: Setting the Course for Next-generation Cancer Neoantigen Discovery for Cancer Vaccines.Genomics, proteomics & bioinformatics · 2021Review
- A Perspective on the Confident Comparison of Glycoprotein Site-Specific Glycosylation in Sample Cohorts.Biochemistry · 2020Review
- Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.Journal of proteome research · 2020Article
- NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.Molecular & cellular proteomics : MCP · 2020Article
- Characterization of glycan isomers using magnetic carbon nanoparticles as a MALDI co-matrix.RSC advances · 2019Article
- Large-Scale Identification and Fragmentation Pathways Analysis of N-Glycans from Mouse Brain.Journal of the American Society for Mass Spectrometry · 2019Article
- A carbon nanoparticles-based solid-phase purification method facilitating sensitive MALDI-MS analysis of permethylated N-glycans.Electrophoresis · 2018Article
- Application of network smoothing to glycan LC-MS profiling.Bioinformatics (Oxford, England) · 2018Article
- Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses.Chemical reviews · 2018Review
- Carbon Nanoparticles and Graphene Nanosheets as MALDI Matrices in Glycomics: a New Approach to Improve Glycan Profiling in Biological Samples.Journal of the American Society for Mass Spectrometry · 2018Article
- Direct comparison of derivatization strategies for LC-MS/MS analysis of N-glycans.The Analyst · 2017Article
- Article
- Isomeric Separation of Permethylated Glycans by Porous Graphitic Carbon (PGC)-LC-MS/MS at High Temperatures.Analytical chemistry · 2017Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- Recent advances in mass spectrometric analysis of glycoproteins.Electrophoresis · 2017Review
- Reversed-phase separation methods for glycan analysis.Analytical and bioanalytical chemistry · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
LC-MS/MS is one of the most powerful tools for N-glycan structure elucidation; however, it is still challenging to identify some glycan structures with low abundance. In this study, we investigated the chromatographic behavior of permethylated N-glycans. The relationship between retention times versus molecular weight of dextran, dextrin, and model glycans was investigated. Also, the nonpolar surface area of glycans was calculated and compared to their experimental retention times. Both retention time and nonpolar surface area trends are similar when the intermolecular interaction is included in the calculation. Moreover, retention time corresponds to glycan types and branch types. The N-glycans analysis model, which combines high mass accuracy and retention time, was applied to confirm serum N-glycans. In total, there were 78 N-glycan compositions identified. A linear relationship between retention times and molecular weights were observed for each subgroup of glycan structures, for example, R(2) value for complex N-glycans was determined to be > 0.98. Moreover, the retention time could be further applied to distinguish between structural isomers as well as linkage isomers. MS/MS data were used to confirm the structural isomers.
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