ArticleElectrophoresis2016
LC-MS/MS analysis of permethylated free oligosaccharides and N-glycans derived from human, bovine, and goat milk samples.
Article in Electrophoresis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 93 citations in OpenAlex.
- Pilot study of probiotic/colostrum supplementation on gut function in children with autism and gastrointestinal symptoms.PloS one · 2019Trial
- Unraveling Cross-Ring Dissociation Mechanisms of Hexoses in Collision-Induced Dissociation.The journal of physical chemistry. A · 2026Article
- Isomeric diversity of GlcNAc(ManScientific reports · 2026Article
- Integrating High-Resolution Cyclic Ion Mobility Separations with Tandem Mass Spectrometry and Collision Cross Section Measurements for Human Milk Oligosaccharide Sequencing.ACS measurement science au · 2025Article
- Unusual free trisaccharides in caprine colostrum discovered by logically derived sequence tandem mass spectrometry.Scientific reports · 2025Article
- Species-specific N-glycan patterns in animal and human breast milk samples.Frontiers in nutrition · 2025Article
- Collision-Induced Dissociations of Linear Hexose and Disaccharides with Linear Hexose at the Reducing End.The journal of physical chemistry. A · 2024Article
- High Abundance of Unusual High MannoseACS omega · 2024Article
- Development of a cyclic ion mobility spectrometry-mass spectrometry-based collision cross-section database of permethylated human milk oligosaccharides.Journal of mass spectrometry : JMS · 2024Article
- Chromatograms and Mass Spectra of High-Mannose and PaucimannoseJournal of proteome research · 2024Article
- Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.Scientific reports · 2023Article
- Identification of the High MannoseAnalytical chemistry · 2023Article
- An Efficient and EconomicalMetabolites · 2022Article
- Heat Stress of Algal Partner Hinders Colonization Success and Alters the Algal Cell Surface Glycome in a Cnidarian-Algal Symbiosis.Microbiology spectrum · 2022Article
- Unusual free oligosaccharides in human bovine and caprine milk.Scientific reports · 2022Article
- Isomeric separation of permethylated glycans by extra-long reversed-phase liquid chromatography (RPLC)-MS/MS.The Analyst · 2022Article
- Salmonella enterica serovar Typhimurium chitinases modulate the intestinal glycome and promote small intestinal invasion.PLoS pathogens · 2022Article
- Food glycomics: Dealing with unexpected degradation of oligosaccharides during sample preparation and analysis.Journal of food and drug analysis · 2022Article
- Review
- Changes in the Expression of Renal Brush Border MembraneBiomolecules · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Oligosaccharides in milk not only provide nutrition to the infants but also have significant immune biofunctions such as inhibition of pathogen binding to the host cell. The main component in milk oligosaccharides is free oligosaccharides. Since the proteins in milk are highly glycosylated, N-glycans in milk also play an import role. In this study, we investigated the permethylated free oligosaccharides and N-glycans extracted from bovine, goat, and human milks using LC-MS/MS. Quantitation profiles of free oligosaccharides and N-glycans were reported. The number of free oligosaccharides observed in bovine, goat, and human milk samples (without isomeric consideration) were 11, 8, and 11, respectively. Human milk had more complex free oligosaccharides structures than the other two milk samples. Totally 58, 21, and 43 N-glycan structures (without isomeric consideration) were associated with whey proteins extracted from bovine, goat, and human milk samples, respectively. Bovine milk free oligosaccharides and N-glycans from whey proteins were highly sialylated and to a lesser extend fucosylated. Goat and human milk free oligosaccharides and N-glycans from whey proteins were both highly fucosylated. Also, the isomeric glycans in milk samples were determined by porous graphitic carbon LC at elevated temperatures. For example, separation of human milk free oligosaccharide Gal-GlcNAc-(Fuc)-Gal-Glc and Gal-GlcNAc-Gal-Glc-Fuc isomers was achieved using porous graphitic carbon column. Permethylation of the glycan structures facilitated the interpretation of MS/MS. For example, internal cleavage and glycosidic bond cleavage are readily distinguished in the tandem mass spectra of permethylated glycans. This feature resulted in the identification of several isomers.
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