ArticleBiophysical journal2016
Intracellular Pressure Dynamics in Blebbing Cells.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- A role for PaxB in regulating blebbing: experimental insights and theoretical perspectives from Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- Cytoplasmic crowding acts as a porous medium reducing macromolecule diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Biophysical modeling identifies an optimal hybrid amoeboid-mesenchymal mechanism for maximal T cell migration speeds.Cell reports · 2026Article
- A role for PaxB in regulating blebbing: experimental insights and theoretical perspectives fromResearch square · 2026Article
- Biophysical modeling identifies an optimal hybrid amoeboid-mesenchymal mechanism for maximal T cell migration speeds.bioRxiv : the preprint server for biology · 2025Article
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Physical principles and mechanisms of cell migration.npj biological physics and mechanics · 2025Review
- Benefits and challenges of reconstituting the actin cortex.Cytoskeleton (Hoboken, N.J.) · 2024Review
- Modeling the dynamics of actin and myosin during bleb stabilization.bioRxiv : the preprint server for biology · 2023Article
- Robotic Intracellular Pressure Measurement Using Micropipette Electrode.Sensors (Basel, Switzerland) · 2023Article
- The Pressure Dependence of the Stability of the G-quadruplex Formed by d(TGGGGT).Life (Basel, Switzerland) · 2022Article
- A reduced 1D stochastic model of bleb-driven cell migration.Biophysical journal · 2022Article
- A role for myosin II clusters and membrane energy in cortex rupture for Dictyostelium discoideum.PloS one · 2022Article
- Article
- Lymphocyte egress signal sphingosine-1-phosphate promotes ERM-guided, bleb-based migration.The Journal of cell biology · 2021Article
- Cell nucleus as a microrheological probe to study the rheology of the cytoskeleton.Biophysical journal · 2021Article
- Differences in creep response of GBM cells migrating in confinement.International biomechanics · 2020Article
- Nonlinear Elastic and Inelastic Properties of Cells.Journal of biomechanical engineering · 2020Review
- Computational estimates of mechanical constraints on cell migration through the extracellular matrix.PLoS computational biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blebs are pressure-driven protrusions that play an important role in cell migration, particularly in three-dimensional environments. A bleb is initiated when the cytoskeleton detaches from the cell membrane, resulting in the pressure-driven flow of cytosol toward the area of detachment and local expansion of the cell membrane. Recent experiments involving blebbing cells have led to conflicting hypotheses regarding the timescale of intracellular pressure propagation. The interpretation of one set of experiments supports a poroelastic model of the cytoplasm that leads to slow pressure equilibration when compared to the timescale of bleb expansion. A different study concludes that pressure equilibrates faster than the timescale of bleb expansion. To address this discrepancy, a dynamic computational model of the cell was developed that includes mechanics of and the interactions among the cytoplasm, the actin cortex, the cell membrane, and the cytoskeleton. The model results quantify the relationship among cytoplasmic rheology, pressure, and bleb expansion dynamics, and provide a more detailed picture of intracellular pressure dynamics. This study shows the elastic response of the cytoplasm relieves pressure and limits bleb size, and that both permeability and elasticity of the cytoplasm determine bleb expansion time. Our model with a poroelastic cytoplasm shows that pressure disturbances from bleb initiation propagate faster than the timescale of bleb expansion and that pressure equilibrates slower than the timescale of bleb expansion. The multiple timescales in intracellular pressure dynamics explain the apparent discrepancy in the interpretation of experimental results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.