Evidence map›Paper›PMID 26958317›Full record

ArticleCartilage2016

Collagen Type IV and Laminin Expressions during Cartilage Repair and in Late Clinically Failed Repair Tissues from Human Subjects.

Casper Bindzus Foldager, Wei Seong Toh, Bjørn Borsøe Christensen, Martin Lind, Andreas H Gomoll, Myron Spector

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cartilage, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Casper Bindzus FoldagerDepartment of Orthopedics, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA; Tissue Engineering Labs, VA Boston Healthcare System, Boston, MA, USA; Orthopaedic Research Laboratory, Aarhus University Hospital, Aarhus, Denmark.
Wei Seong TohDepartment of Orthopedics, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA; Tissue Engineering Labs, VA Boston Healthcare System, Boston, MA, USA; Discipline of Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore.
Bjørn Borsøe ChristensenOrthopaedic Research Laboratory, Aarhus University Hospital, Aarhus, Denmark.
Martin LindSports Trauma Clinic, Department of Orthopaedics, Aarhus University Hospital, Aarhus, Denmark.
Andreas H GomollCartilage Repair Center, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
Myron SpectorDepartment of Orthopedics, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA; Tissue Engineering Labs, VA Boston Healthcare System, Boston, MA, USA.
Aarhus University Hospital · DKBrigham and Women's Hospital · USHarvard University · USVA Boston Healthcare System · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo identify the collagen type IV (Col4) isoform in articular cartilage and to evaluate the expressions of Col4 and laminin in the pericellular matrix (PCM) in damaged cartilage and during cartilage repair.

designThe Col4 isoform was determined in chondrocytes isolated from 6 patients cultured up to 6 days and in 21% O2 or 1% O2, and the gene expression of Col4 α-chains was investigated. The distribution of Col4 and laminin in traumatically damaged cartilage (n = 7) and clinically failed cartilage repair (microfracture, TruFit, autologous chondrocyte implantation; n = 11) were investigated using immunohistochemistry. Normal human cartilage was used as control (n = 8). The distribution during clinical cartilage repair procedures was investigated in a minipig model with 6-month follow-up (untreated chondral, untreated osteochondral, microfracture, autologous chondrocyte implantation; n = 10).

resultsThe Col4 isoform in articular cartilage was characterized as α1α1α2, which is an isoform containing antiangiogenic domains in the NC1-terminals (arresten and canstatin). In normal cartilage, laminin and Col4 was exclusively found in the PCM. High amounts (>50%) of Col4 in the PCM significantly decreased in damaged cartilage (P = 0.004) and clinically failed repair tissue (P < 0.001). Laminin was only found with high expression (>50%) in 4/8 of the normal samples, which was not statistically significantly different from damaged cartilage (P = 0.15) or failed cartilage repair (P = 0.054).

conclusionsCol4 in cartilage contain antiangiogenic domains and may play a role in the hypoxic environment in articular cartilage. Col4 and laminin was not found in the PCM of damaged and clinically failed repair.

Indexed as

articular cartilagecartilage repaircollagen type IVlaminin

Identifiers

PMID26958317
PMCPMC4749752
OpenAlexW2341819712

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.