ArticleBiomicrofluidics2016
Quantitative analysis of the chemotaxis of a green alga, Chlamydomonas reinhardtii, to bicarbonate using diffusion-based microfluidic device.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Drift velocity of bacterial chemotaxis in dynamic chemical environments.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2025Article
- Preparation, maintenance and propagation of synchronous cultures of photoactive Chlamydomonas cells.Nature protocols · 2025Review
- Microalgae-driven microrobots: revolutionizing drug delivery and targeted therapy in biopharmaceuticals.Advanced biotechnology · 2025Review
- A structural perspective on insect gustatory receptors.Chemical senses · 2025Review
- COP5/HKR1 changes ciliary beat pattern and biases cell steering during chemotaxis in Chlamydomonas reinhardtii.Scientific reports · 2024Article
- The younger flagellum sets the beat foreLife · 2024Article
- Cells collectively migrate during ammonium chemotaxis in Chlamydomonas reinhardtii.Scientific reports · 2023Article
- Improved Productivity of Astaxanthin from PhotosensitiveMarine drugs · 2022Article
- Augmented CONature communications · 2021Article
- Enhancement of microalgae growth using magnetic artificial cilia.Biotechnology and bioengineering · 2021Article
- Advances in understanding the physiological role and locations of carbonic anhydrases in C3 plant cells.Protoplasma · 2021Review
- Insights on the Functions and Ecophysiological Relevance of the Diverse Carbonic Anhydrases in Microalgae.International journal of molecular sciences · 2020Review
- Elucidation and genetic intervention of COJournal of biosciences · 2020Article
- The BBSome restricts entry of tagged carbonic anhydrase 6 into the cis-flagellum of Chlamydomonas reinhardtii.PloS one · 2020Article
- Cilia-based peptidergic signaling.PLoS biology · 2019Article
- Multiomics resolution of molecular events during a day in the life of Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Microfluidic technology for plankton research.Current opinion in biotechnology · 2019Review
- An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.Metabolites · 2018Review
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a growing interest in the photosynthetic carbon fixation by microalgae for the production of valuable products from carbon dioxide (CO2). Microalgae are capable of transporting bicarbonate (HCO3 (-)), the most abundant form of inorganic carbon species in the water, as a source of CO2 for photosynthesis. Despite the importance of HCO3 (-) as the carbon source, little is known about the chemotactic response of microalgae to HCO3 (-). Here, we showed the chemotaxis of a model alga, Chlamydomonas reinhardtii, towards HCO3 (-) using an agarose gel-based microfluidic device with a flow-free and stable chemical gradient during the entire assay period. The device was validated by analyzing the chemotactic responses of C. reinhardtii to the previously known chemoattractants (NH4Cl and CoCl2) and chemotactically neutral molecule (NaCl). We found that C. reinhardtii exhibited the strongest chemotactic response to bicarbonate at the concentration of 26 mM in a microfluidic device. The chemotactic response to bicarbonate showed a circadian rhythm with a peak during the dark period and a valley during the light period. We also observed the changes in the chemotaxis to bicarbonate by an inhibitor of bicarbonate transporters and a mutation in CIA5, a transcriptional regulator of carbon concentrating mechanism, indicating the relationship between chemotaxis to bicarbonate and inorganic carbon metabolism in C. reinhardtii. To the best of our knowledge, this is the first report of the chemotaxis of C. reinhardtii towards HCO3 (-), which contributes to the understanding of the physiological role of the chemotaxis to bicarbonate and its relevance to inorganic carbon utilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.