ArticleBiomicrofluidics2016
A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Automated Platforms inMicromachines · 2025Review
- Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo.Microsystems & nanoengineering · 2025Article
- Deep Learning for Microfluidic-AssistedMicromachines · 2023Article
- Microinjection inBiomicrofluidics · 2023Article
- Microfluidic-AssistedCyborg and bionic systems (Washington, D.C.) · 2023Article
- 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy.Nature communications · 2021Article
- A hybrid microfluidic device for on-demand orientation and multidirectional imaging ofBiomicrofluidics · 2016Article
- An automated system for high-throughput generation and optimization of microdroplets.Biomicrofluidics · 2016Article
- Microfluidic Approaches for Manipulating, Imaging, and Screening C. elegans.Micromachines · 2016Review
- Preface to Special Topic: Selected Papers from the 5th International Conference on Optofluidics.Biomicrofluidics · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nematode worm Caenorhabditis elegans has been widely used as a model organism in biological studies because of its short and prolific life cycle, relatively simple body structure, significant genetic overlap with human, and facile/inexpensive cultivation. Microinjection, as an established and versatile tool for delivering liquid substances into cellular/organismal objects, plays an important role in C. elegans research. However, the conventional manual procedure of C. elegans microinjection is labor-intensive and time-consuming and thus hinders large-scale C. elegans studies involving microinjection of a large number of C. elegans on a daily basis. In this paper, we report a novel microfluidic device that enables, for the first time, fully automated, high-speed microinjection of C. elegans. The device is automatically regulated by on-chip pneumatic valves and allows rapid loading, immobilization, injection, and downstream sorting of single C. elegans. For demonstration, we performed microinjection experiments on 200 C. elegans worms and demonstrated an average injection speed of 6.6 worm/min (average worm handling time: 9.45 s/worm) and a success rate of 77.5% (post-sorting success rate: 100%), both much higher than the performance of manual operation (speed: 1 worm/4 min and success rate: 30%). We conducted typical viability tests on the injected C. elegans and confirmed that the automated injection system does not impose significant adverse effect on the physiological condition of the injected C. elegans. We believe that the developed microfluidic device holds great potential to become a useful tool for facilitating high-throughput, large-scale worm biology research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.