ArticleGenome research2016
2C-Cas9: a versatile tool for clonal analysis of gene function.
Article in Genome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- All-in-one, Cas13d-based cell-specific gene knockdown system for zebrafish.bioRxiv : the preprint server for biology · 2026Article
- Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.The Journal of cell biology · 2026Article
- Article
- Lineage labeling with zebrafish hand2 Cre and CreERT2 recombinase CRISPR knock-ins.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Post-synaptic density proteins in oligodendrocytes are required for activity-dependent myelin sheath growth.iScience · 2025Article
- EFEMP1 contributes to light-dependent ocular growth in zebrafish.Biology open · 2024Article
- Lineage labeling with zebrafishbioRxiv : the preprint server for biology · 2024Article
- Article
- A noninvasive photoactivatable split-Cre recombinase system for genome engineering in zebrafish.iScience · 2024Article
- Synaptic input and CaNature neuroscience · 2024Article
- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations Via a tRNA-Based Multiplex sgRNA Expression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Thalamic regulation of a visual critical period and motor behavior.Cell reports · 2023Article
- Review
- Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration.Science advances · 2021Article
- A Time-Saving Strategy to Generate Double Maternal Mutants by an Oocyte-Specific Conditional Knockout System in Zebrafish.Biology · 2021Article
- Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish.Science advances · 2021Article
- Transient, flexible gene editing in zebrafish neutrophils and macrophages for determination of cell-autonomous functions.Disease models & mechanisms · 2021Article
- A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish.Journal of cell science · 2021Article
- Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.International journal of molecular sciences · 2020Review
- Excess centrosomes disrupt vascular lumenization and endothelial cell adherens junctions.Angiogenesis · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date, this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study of the function of certain loci might require tight spatiotemporal control of gene inactivation. Here, we show that tissue-specific gene disruption can be achieved by driving Cas9 expression with the Gal4/UAS system. Furthermore, by combining the Gal4/UAS and Cre/loxP systems, we establish a versatile tool to genetically label mutant cell clones, enabling their phenotypic analysis. Our technique has the potential to be applied to diverse model organisms, enabling tissue-specific loss-of-function and phenotypic characterization of live and fixed tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.