ArticleScientific reports2016
Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Optimization of bacterial colonization in the gut of axenic and conventional zebrafish larvae using live food.Microbiology spectrum · 2026Article
- Chemical interactions in polyethylene glycol-induced condensates lead to an anomalous FRET response from a flexible linker-fluorescent protein crowding sensor.bioRxiv : the preprint server for biology · 2026Article
- Article
- Probing the photophysical properties of fluorescent proteins using photoacoustic pump-probe spectroscopy and imaging.Photoacoustics · 2025Article
- Trans-scale live-imaging of an E5.5 mouse embryo using incubator-type biaxial light-sheet microscopy.Life science alliance · 2025Article
- Analysis of Fluorescent Proteins for Observing Single Gene Locus in a Live and FixedThe journal of physical chemistry. B · 2024Article
- Orientation-Independent-DIC imaging reveals that a transient rise in depletion force contributes to mitotic chromosome condensation.bioRxiv : the preprint server for biology · 2024Article
- Intracellular dry mass density increases under growth-induced pressure.Open research Europe · 2024Article
- Increased intracellular crowding during hyperosmotic stress.Scientific reports · 2023Article
- Unlocking chloride sensing in the red at physiological pH with a fluorescent rhodopsin-based host.Chemical communications (Cambridge, England) · 2023Article
- An Image Analysis Pipeline for Quantifying the Features of Fluorescently-Labeled Biomolecular Condensates in Cells.Frontiers in bioinformatics · 2022Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Glycine insertion modulates the fluorescence properties ofBiophysics and physicobiology · 2021Article
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Time-dependent enhancement of fluorescence from Rhodobacter capsulatus SB1003 and its critical dependence on concentration temperature and static magnetic field.Journal of biological physics · 2020Article
- Quantitative in vivo imaging of neuronal glucose concentrations with a genetically encoded fluorescence lifetime sensor.Journal of neuroscience research · 2019Article
- Enhancing fluorescent protein photostability through robot-assisted photobleaching.Integrative biology : quantitative biosciences from nano to macro · 2018Article
- Conformational plasticity of the intrinsically disordered protein ASR1 modulates its function as a drought stress-responsive gene.PloS one · 2018Article
- Efficient Bioimaging with Diaminodicyanoquinodimethanes: Selective Imaging of Epidermal and Stomatal Cells and Insight into the Molecular Level Interactions.Scientific reports · 2017Article
- Crystallization-induced emission enhancement: A novel fluorescent Au-Ag bimetallic nanocluster with precise atomic structure.Science advances · 2017Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent proteins have been widely used in biology because of their compatibility and varied applications in living specimens. Fluorescent proteins are often undesirably sensitive to intracellular conditions such as pH and ion concentration, generating considerable issues at times. However, harnessing these intrinsic sensitivities can help develop functional probes. In this study, we found that the fluorescence of yellow fluorescent protein (YFP) depends on the protein concentration in the solution and that this dependence can be enhanced by adding a glycine residue in to the YFP; we applied this finding to construct an intracellular protein-crowding sensor. A Förster resonance energy transfer (FRET) pair, involving a cyan fluorescent protein (CFP) insensitive to protein concentration and a glycine-inserted YFP, works as a genetically encoded probe to evaluate intracellular crowding. By measuring the fluorescence of the present FRET probe, we were able to detect dynamic changes in protein crowding in living cells.
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