ArticleJournal of mass spectrometry : JMS2016
Travelling-wave ion mobility and negative ion fragmentation of high-mannose N-glycans.
Article in Journal of mass spectrometry : JMS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Effects of Different Adduct Ions, Ionization Temperatures, and Solvents on the Ion Mobility of Glycans.Molecules (Basel, Switzerland) · 2025Article
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.Analytical and bioanalytical chemistry · 2021Article
- Identification of N-glycans with GalNAc-containing antennae from recombinant HIV trimers by ion mobility and negative ion fragmentation.Analytical and bioanalytical chemistry · 2021Article
- State-of-the-Art Glycomics Technologies in Glycobiotechnology.Advances in biochemical engineering/biotechnology · 2021Article
- Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.Analytical chemistry · 2020Article
- Preparative scale purification of natural glycans by closed-loop recycle HPLC.Analytical biochemistry · 2020Article
- Advancing Solutions to the Carbohydrate Sequencing Challenge.Journal of the American Chemical Society · 2019Review
- Separation of Sialylated Glycan Isomers by Differential Mobility Spectrometry.Analytical chemistry · 2019Article
- Ion Mobility Spectrometry and the Omics: Distinguishing Isomers, Molecular Classes and Contaminant Ions in Complex Samples.Trends in analytical chemistry : TRAC · 2019Article
- Sequence Ion Structures and Dissociation Chemistry of Deprotonated Sucrose Anions.Journal of the American Society for Mass Spectrometry · 2018Article
- Structural Studies of Fucosylated N-Glycans by Ion Mobility Mass Spectrometry and Collision-Induced Fragmentation of Negative Ions.Journal of the American Society for Mass Spectrometry · 2018Article
- Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.Journal of the American Society for Mass Spectrometry · 2018Article
- Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.Current opinion in chemical biology · 2018Review
- Glycosylation profiling to evaluate glycoprotein immunogens against HIV-1.Expert review of proteomics · 2017Review
- Structural principles controlling HIV envelope glycosylation.Current opinion in structural biology · 2017Review
- Global N-Glycan Site Occupancy of HIV-1 gp120 by Metabolic Engineering and High-Resolution Intact Mass Spectrometry.ACS chemical biology · 2017Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- Travelling-wave ion mobility mass spectrometry and negative ion fragmentation of hybrid and complex N-glycans.Journal of mass spectrometry : JMS · 2016Article
- Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
The isomeric structure of high-mannose N-glycans can significantly impact biological recognition events. Here, the utility of travelling-wave ion mobility mass spectrometry for isomer separation of high-mannose N-glycans is investigated. Negative ion fragmentation using collision-induced dissociation gave more informative spectra than positive ion spectra with mass-different fragment ions characterizing many of the isomers. Isomer separation by ion mobility in both ionization modes was generally limited, with the arrival time distributions (ATD) often showing little sign of isomers. However, isomers could be partially resolved by plotting extracted fragment ATDs of the diagnostic fragment ions from the negative ion spectra, and the fragmentation spectra of the isomers could be extracted by using ions from limited areas of the ATD peak. In some cases, asymmetric ATDs were observed, but no isomers could be detected by fragmentation. In these cases, it was assumed that conformers or anomers were being separated. Collision cross sections of the isomers in positive and negative fragmentation mode were estimated from travelling-wave ion mobility mass spectrometry data using dextran glycans as calibrant. More complete collision cross section data were achieved in negative ion mode by utilizing the diagnostic fragment ions. Examples of isomer separations are shown for N-glycans released from the well-characterized glycoproteins chicken ovalbumin, porcine thyroglobulin and gp120 from the human immunodeficiency virus. In addition to the cross-sectional data, details of the negative ion collision-induced dissociation spectra of all resolved isomers are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.