ArticleRice (New York, N.Y.)2016
Heterologous Expression and Functional Analysis of Rice GLUTAMATE RECEPTOR-LIKE Family Indicates its Role in Glutamate Triggered Calcium Flux in Rice Roots.
Article in Rice (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 43 citations in OpenAlex.
- Genetic Dissection of Rice Plant-Specific Non-ε Group 14-3-3 Proteins in Response to Phosphate Starvation.Rice (New York, N.Y.) · 2025Article
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- Rice NLR protein XinN1, induced by a pattern recognition receptor XA21, confers enhanced resistance to bacterial blight.Plant cell reports · 2024Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Merging Signaling with Structure: Functions and Mechanisms of Plant Glutamate Receptor Ion Channels.Annual review of plant biology · 2023Review
- Biotic stress-induced changes in root exudation confer plant stress tolerance by altering rhizospheric microbial community.Frontiers in plant science · 2023Review
- Comprehensive Analysis of Glutamate Receptor-like Genes in Rice (Current issues in molecular biology · 2022Article
- Roles of Glutamate Receptor-Like Channels (GLRs) in Plant Growth and Response to Environmental Stimuli.Plants (Basel, Switzerland) · 2022Review
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Genome-Wide Identification, Characterization, and Expression Analysis of Glutamate Receptor-like Gene (Plants (Basel, Switzerland) · 2022Article
- Wound-Induced Systemic Responses and Their Coordination by Electrical Signals.Frontiers in plant science · 2022Review
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- A Single-Nucleotide Mutation in a GLUTAMATE RECEPTOR-LIKE Gene Confers Resistance to Fusarium Wilt inAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Review
- Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays.Plant methods · 2020Article
- Reducing Cadmium Accumulation in Plants: Structure-Function Relations and Tissue-Specific Operation of Transporters in the Spotlight.Plants (Basel, Switzerland) · 2020Review
- Root Exudation of Primary Metabolites: Mechanisms and Their Roles in Plant Responses to Environmental Stimuli.Frontiers in plant science · 2019Review
- The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root.Journal of experimental botany · 2018Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTremendous progress has been made in understanding the functions of the GLUTAMATE RECEPTOR-LIKE (GLR) family in Arabidopsis. Still, the functions of OsGLRs in rice, especially the ion channel activities, are largely unknown.
resultsUsing the aequorin-based luminescence imaging system, we screened the specificity of amino acids involved in the induction of Ca(2+) flux in rice roots. Of all the amino acids tested, glutamate (Glu) was the only one to trigger Ca(2+) flux significantly in rice roots. Detailed analysis showed a dose response of Ca(2+) increase to different concentrations of Glu. In addition, the Ca(2+) spike response to Glu was rapid, within 20 s after the application. A desensitization assay and pharmacological tests showed that the Glu-triggered Ca(2+) flux is mediated by OsGLRs. Whole genome analysis identified 13 OsGLR genes in rice, and these genes have various expression patterns in different tissues. Subcellular localization studies showed that all the OsGLRs examined are likely localized to the plasma membrane. Bacteria growth assays showed that at least OsGLR2.1 and OsGLR3.2 have the potential to mediate ion uptake in bacteria. Further analysis using Fura-2-based Ca(2+) imaging revealed a Glu-triggered Ca(2+) increase in OsGLR2.1-expressing human embryonic kidney (HEK) cells.
conclusionsOur work provides a molecular basis for investigating mechanisms of Glu-triggered Ca(2+) flux in rice.
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