ArticleAmerican journal of physiology. Endocrinology and metabolism2016
Estradiol modulates myosin regulatory light chain phosphorylation and contractility in skeletal muscle of female mice.
Article in American journal of physiology. Endocrinology and metabolism, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 71 citations in OpenAlex.
- Dietary nitrate and muscle contractile function in women: effect of menstrual cycle phase.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Trial
- Shatavari Supplementation in Postmenopausal Women Improves Handgrip Strength and IncreasesNutrients · 2021Trial
- Serial sarcomere adaptation to eccentric overload training is blunted in ovariectomized rats.Physiological reports · 2026Article
- Alterations to longitudinal muscle morphology and mechanical function following immobilization and recovery in female ovariectomized and intact rats.Experimental physiology · 2026Article
- Loss of ovarian hormones modulates sex-specific metabolic adaptations to volumetric muscle loss.American journal of physiology. Cell physiology · 2026Article
- Sex Hormones & Exercise in the Musculoskeletal System: From Signaling to Structure and Function.Endocrine reviews · 2026Article
- Exercise-induced mitochondrial protection in skeletal muscle of ovariectomized mice: A myogenic ERedox biology · 2025Article
- Ovarian Aging: The Silent Catalyst of Age-Related Disorders in Female Body.Aging and disease · 2025Review
- Fatiguing exercise reduces cellular passive Young's modulus in human vastus lateralis muscle.Experimental physiology · 2024Article
- Time course changes in in vivo muscle mechanical function and CaExperimental physiology · 2024Article
- Ablation of skeletal muscle estrogen receptor alpha impairs contractility in male mice.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Estrogen deficiency reduces maximal running capacity and affects serotonin levels differently in the hippocampus and nucleus accumbens in response to acute exercise.Frontiers in neuroscience · 2024Article
- Passive exercise is an effective alternative to HRT for restoring OVX induced mitochondrial dysfunction in skeletal muscle.Frontiers in endocrinology · 2024Article
- Estrogen Status Influences Whole-Body Vibration Training-Induced Improvements on Muscle Mass and Strength in Female Ovariectomized Mice.International journal of medical sciences · 2024Article
- Article
- The asymmetrical ESR1 signaling in muscle progenitor cells determines the progression of adolescent idiopathic scoliosis.Cell discovery · 2023Article
- In vivo potentiation of muscle torque is enhanced in female mice through estradiol-estrogen receptor signaling.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Sex-Related Effects of Gut Microbiota in Metabolic Syndrome-Related Diabetic Retinopathy.Microorganisms · 2023Review
- Mechanisms of Estrogen Influence on Skeletal Muscle: Mass, Regeneration, and Mitochondrial Function.Sports medicine (Auckland, N.Z.) · 2022Review
- Global phosphoproteomic profiling of skeletal muscle in ovarian hormone-deficient mice.Physiological genomics · 2022Article
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Authors and funding
5 authors at 2 institutions in 3 countries.
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Abstract
Impairment of skeletal muscle function has been associated with changes in ovarian hormones, especially estradiol. To elucidate mechanisms of estradiol on skeletal muscle strength, the hormone's effects on phosphorylation of the myosin regulatory light chain (pRLC) and muscle contractility were investigated, hypothesizing an estradiol-specific beneficial impact. In a skeletal muscle cell line, C2C12, pRLC was increased by 17β-estradiol (E2) in a concentration-dependent manner. In skeletal muscles of C57BL/6 mice that were E2 deficient via ovariectomy (OVX), pRLC was lower than that from ovary-intact, sham-operated mice (Sham). The reduced pRLC in OVX muscle was reversed by in vivo E2 treatment. Posttetanic potentiation (PTP) of muscle from OVX mice was low compared with that from Sham mice, and this decrement was reversed by acute E2 treatment, demonstrating physiological consequence. Western blot of those muscles revealed that low PTP corresponded with low pRLC and higher PTP with greater pRLC. We aimed to elucidate signaling pathways affecting E2-mediated pRLC using a kinase inhibitor library and C2C12 cells as well as a specific myosin light chain kinase inhibitor in muscles. PI3K/Akt, MAPK, and CamKII were identified as candidate kinases sensitive to E2 in terms of phosphorylating RLC. Applying siRNA strategy in C2C12 cells, pRLC triggered by E2 was found to be mediated by estrogen receptor-β and the G protein-coupled estrogen receptor. Together, these results provide evidence that E2 modulates myosin pRLC in skeletal muscle and is one mechanism by which this hormone can affect muscle contractility in females.
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