ArticlePloS one2016
Effects of Selected Egyptian Honeys on the Cellular Ultrastructure and the Gene Expression Profile of Escherichia coli.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
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- Investigation of antimicrobial and antivirulence effects of lactobionic acid and carvacrol, individually and in combination, against Escherichia coli isolates.Molecular biology reports · 2026Article
- Bioactive Dressing: A New Algorithm in Wound Healing.Journal of clinical medicine · 2024Review
- Comparison of the antimicrobial and antivirulence activities of Sidr and Tualang honeys with Manuka honey againstIranian journal of microbiology · 2023Article
- ComparativeAIMS microbiology · 2023Article
- The New Strategy for Studying Drug-Delivery Systems with Prolonged Release: Seven-Day In Vitro Antibacterial Action.Molecules (Basel, Switzerland) · 2022Article
- Honey dressing: a missed way for orthopaedic wound care.International orthopaedics · 2022Article
- A comparative study of antibacterial and antivirulence activities of four selected honeys to Manuka honey.Iranian journal of microbiology · 2022Article
- Role of Honey in Advanced Wound Care.Molecules (Basel, Switzerland) · 2021Review
- Honey as an Ecological Reservoir of Antibacterial Compounds Produced by Antagonistic Microbial Interactions in Plant Nectars, Honey and Honey Bee.Antibiotics (Basel, Switzerland) · 2021Review
- Evolution of honey resistance in experimental populations of bacteria depends on the type of honey and has no major side effects for antibiotic susceptibility.Evolutionary applications · 2021Article
- Use of Transposon Directed Insertion-Site Sequencing to Probe the Antibacterial Mechanism of a Model Honey onFrontiers in microbiology · 2021Article
- Antibacterial properties of selected Malaysian Tualang honey againstIranian journal of microbiology · 2020Article
- Honey: Another Alternative in the Fight against Antibiotic-Resistant Bacteria?Antibiotics (Basel, Switzerland) · 2020Review
- Article
- The ultrastructural damage caused by Eugenia zeyheri and Syzygium legatii acetone leaf extracts on pathogenic Escherichia coli.BMC veterinary research · 2020Article
- Article
- The combined antimicrobial activity of citrus honey and fosfomycin on multidrug resistantAIMS microbiology · 2020Article
- The anti-virulence effect of cranberry active compound proanthocyanins (PACs) on expression of genes in the third-generation cephalosporin-resistantAntimicrobial resistance and infection control · 2019Article
- Potential antibacterial activity of some Saudi Arabia honey.Veterinary world · 2017Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to: (i) evaluate the antibacterial activities of three Egyptian honeys collected from different floral sources (namely, citrus, clover, and marjoram) against Escherichia coli; (ii) investigate the effects of these honeys on bacterial ultrastructure; and (iii) assess the anti-virulence potential of these honeys, by examining their impacts on the expression of eight selected genes (involved in biofilm formation, quorum sensing, and stress survival) in the test organism. The minimum inhibitory concentration (MIC) of the honey samples against E. coli ATCC 8739 were assessed by the broth microdilution assay in the presence and absence of catalase enzyme. Impacts of the honeys on the cellular ultrastructure and the expression profiles of the selected genes of E. coli were examined using transmission electron microscopy (TEM) and quantitative real-time polymerase chain reaction (qPCR) analysis, respectively. The susceptibility tests showed promising antibacterial activities of all the tested honeys against E. coli. This was supported by the TEM observations, which revealed "ghost" cells lacking DNA, in addition to cells with increased vacuoles, and/or with irregular shrunken cytoplasm. Among the tested honeys, marjoram exhibited the highest total antibacterial activity and the highest levels of peroxide-dependent activity. The qPCR analysis showed that all honey-treated cells share a similar overall pattern of gene expression, with a trend toward reduced expression of the virulence genes of interest. Our results indicate that some varieties of the Egyptian honey have the potential to be effective inhibitor and virulence modulator of E. coli via multiple molecular targets.
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