ArticlePloS one2016
Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Neurological Inflammation in Parkinsonism: Current Prognosticative Diagnostics and Pitfalls.Current topics in medicinal chemistry · 2025Review
- Establishment of a Quenchbody-based L-thyroxine detection method and its comparison with ELISA systems.Analytical and bioanalytical chemistry · 2024Article
- Tau biosensor on aptamer-modified interdigitated electrode for monitoring neurological effect caused by anesthesia.Heliyon · 2024Article
- Quantum Dot-Based Nanosensors for In Vitro Detection ofNanomaterials (Basel, Switzerland) · 2024Review
- Modified streptavidin-biotin based lateral flow test strip for rapid detection of SARS-CoV-2 S1 antigen in saliva samples.Scientific reports · 2024Article
- MicroLED biosensor with colloidal quantum dots and smartphone detection.Biomedical optics express · 2023Article
- On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen.Mikrochimica acta · 2022Article
- Immobilization of Streptavidin on a Plasmonic Au-TiONanomaterials (Basel, Switzerland) · 2022Article
- Nanowired dual-electrodes surface to monitor cerebral ischemia by current-volt measurements.3 Biotech · 2021Article
- Zeolite-iron oxide nanocomposite from fly ash formed a 'clubbell' structure: integration of cardiac biocapture macromolecules in serum on microelectrodes.Mikrochimica acta · 2021Article
- An iron oxide nanoworm hybrid on an interdigitated microelectrode silica surface to detect abdominal aortic aneurysms.Mikrochimica acta · 2021Article
- An Automatic Platform Based on Nanostructured Microfluidic Chip for Isolating and Identification of Circulating Tumor Cells.Micromachines · 2021Article
- Electrically Stimulated Tunable Drug Delivery From Polypyrrole-Coated Polyvinylidene Fluoride.Frontiers in chemistry · 2021Article
- Electroanalysis on an Interdigitated Electrode for High-Affinity Cardiac Troponin I Biomarker Detection by Aptamer-Gold Conjugates.ACS omega · 2020Article
- Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity.Biosensors & bioelectronics · 2020Article
- Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC biotechnology · 2020Article
- Carbon-Coated Superparamagnetic Nanoflowers for Biosensors Based on Lateral Flow Immunoassays.Biosensors · 2020Article
- Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay.Nanomaterials (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzyme Linked Immunosorbent Assay (ELISA) is the gold standard assay for detecting and identifying biomolecules using antibodies as the probe. Improving ELISA is crucial for detecting disease-causing agents and facilitating diagnosis at the early stages of disease. Biotinylated antibody and streptavidin-conjugated horse radish peroxide (streptavidin-HRP) often are used with ELISA to enhance the detection of various kinds of targets. In the present study, we used a competition-based strategy in which we pre-mixed free biotin with streptavidin-HRP to generate high-performance system, as free biotin occupies some of the biotin binding sites on streptavidin, thereby providing more chances for streptavidin-HRP to bind with biotinylated antibody. ESAT-6, which is a protein secreted early during tuberculosis infection, was used as the model target. We found that 8 fM of free biotin mixed with streptavidin-HRP anchored the higher detection level of ESAT-6 by four-fold compared with detection without free biotin (only streptavidin-HRP), and the limit of detection of the new method was 250 pM. These results suggest that biotin-streptavidin competition can be used to improve the diagnosis of analytes in other types of sensors.
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