ArticleThe ISME journal2016
Local diversity of heathland Cercozoa explored by in-depth sequencing.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Land use reshapes soil cercozoan functional architecture through soil chemistry and fungal networks.ISME communications · 2026Article
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- Metabarcoding of protozoa and helminth in black-necked cranes: a high prevalence of parasites and free-living amoebae.Parasite (Paris, France) · 2024Article
- Abrupt permafrost thaw triggers activity of copiotrophs and microbiome predators.FEMS microbiology ecology · 2023Article
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- Information Scale Correction for Varying Length Amplicons Improves Eukaryotic Microbiome Data Integration.Microorganisms · 2023Article
- Community dynamics of microbial eukaryotes in intertidal mudflats in the hypertidal Bay of Fundy.ISME communications · 2023Article
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- The Diversity Patterns of Rare to Abundant Microbial Eukaryotes Across a Broad Range of Salinities in a Solar Saltern.Microbial ecology · 2022Article
- The combined impact of low temperatures and shifting phosphorus availability on the competitive ability of cyanobacteria.Scientific reports · 2022Article
- Ecological clusters of soil taxa within bipartite networks are highly sensitive to climatic conditions in global drylands.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Water deficit affects inter-kingdom microbial connections in plant rhizosphere.Environmental microbiology · 2022Article
- Does filter pore size introduce bias in DNA sequence-based plankton community studies?Frontiers in microbiology · 2022Article
- On the phenology of protists: recurrent patterns reveal seasonal variation of protistan (Rhizaria: Cercozoa and Endomyxa) communities in tree canopies.FEMS microbiology ecology · 2021Article
- Soil microbial legacies differ following drying-rewetting and freezing-thawing cycles.The ISME journal · 2021Article
- Taxonomic and Functional Diversity of Heterotrophic Protists (Cercozoa and Endomyxa) from Biological Soil Crusts.Microorganisms · 2021Article
- What Drives the Diversity of the Most Abundant Terrestrial Cercozoan Family (Rhogostomidae, Cercozoa, Rhizaria)?Microorganisms · 2020Article
- Rain-Fed Granite Rock Basins Accumulate a High Diversity of Dormant Microbial Eukaryotes.Microbial ecology · 2020Article
- Comparative analyses of the V4 and V9 regions of 18S rDNA for the extant eukaryotic community using the Illumina platform.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cercozoa are abundant free-living soil protozoa and quantitatively important in soil food webs; yet, targeted high-throughput sequencing (HTS) has not yet been applied to this group. Here we describe the development of a targeted assay to explore Cercozoa using HTS, and we apply this assay to measure Cercozoan community response to drought in a Danish climate manipulation experiment (two sites exposed to artificial drought, two unexposed). Based on a comparison of the hypervariable regions of the 18S ribosomal DNA of 193 named Cercozoa, we concluded that the V4 region is the most suitable for group-specific diversity analysis. We then designed a set of highly specific primers (encompassing ~270 bp) for 454 sequencing. The primers captured all major cercozoan groups; and >95% of the obtained sequences were from Cercozoa. From 443 350 high-quality short reads (>300 bp), we recovered 1585 operational taxonomic units defined by >95% V4 sequence similarity. Taxonomic annotation by phylogeny enabled us to assign >95% of our reads to order level and ~85% to genus level despite the presence of a large, hitherto unknown diversity. Over 40% of the annotated sequences were assigned to Glissomonad genera, whereas the most common individually named genus was the euglyphid Trinema. Cercozoan diversity was largely resilient to drought, although we observed a community composition shift towards fewer testate amoebae.
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