ArticleThe ISME journal2016
Biofilm plasmids with a rhamnose operon are widely distributed determinants of the 'swim-or-stick' lifestyle in roseobacters.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 78 citations in OpenAlex.
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- Olivine and dissolved alkalinity trigger different bacterial community shifts in water and oyster gills: insights from a mesocosm experiment.Frontiers in microbiomes · 2025Article
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- L-Rhamnose Globally Changes the Transcriptome of Planktonic and BiofilmMicroorganisms · 2024Article
- Different Roles of Dioxin-Catabolic Plasmids in Growth, Biofilm Formation, and Metabolism ofMicroorganisms · 2024Article
- Genomic and induction evidence for bacteriophage contributions to sargassum-bacteria symbioses.Microbiome · 2024Article
- Genomic Analysis of NovelMarine drugs · 2024Article
- Co-evolution of gene transfer agents and their alphaproteobacterial hosts.Journal of bacteriology · 2024Article
- Novel metabolite madeirone and neomarinone extracted fromFrontiers in chemistry · 2024Article
- Marine particle size-fractionation indicates organic matter is processed by differing microbial communities on depth-specific particles.ISME communications · 2024Article
- Biofilm formation stabilizes metabolism in aApplied and environmental microbiology · 2023Article
- Epilithic Bacterial Assemblages on Subtidal Rocky Reefs: Variation Among Alternative Habitats at Ambient and Enhanced Nutrient Levels.Microbial ecology · 2023Article
- Copiotrophy in a Marine-Biofilm-Derived Roseobacteraceae Bacterium Can Be Supported by Amino Acid Metabolism and Thiosulfate Oxidation.International journal of molecular sciences · 2023Article
- Anaerobic thiosulfate oxidation by the Roseobacter group is prevalent in marine biofilms.Nature communications · 2023Article
- Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China.Microbiology spectrum · 2022Article
- Ecogenomics reveals viral communities across the Challenger Deep oceanic trench.Communications biology · 2022Article
- The Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae.mSystems · 2022Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Alphaproteobacteria of the metabolically versatile Roseobacter group (Rhodobacteraceae) are abundant in marine ecosystems and represent dominant primary colonizers of submerged surfaces. Motility and attachment are the prerequisite for the characteristic 'swim-or-stick' lifestyle of many representatives such as Phaeobacter inhibens DSM 17395. It has recently been shown that plasmid curing of its 65-kb RepA-I-type replicon with >20 genes for exopolysaccharide biosynthesis including a rhamnose operon results in nearly complete loss of motility and biofilm formation. The current study is based on the assumption that homologous biofilm plasmids are widely distributed. We analyzed 33 roseobacters that represent the phylogenetic diversity of this lineage and documented attachment as well as swimming motility for 60% of the strains. All strong biofilm formers were also motile, which is in agreement with the proposed mechanism of surface attachment. We established transposon mutants for the four genes of the rhamnose operon from P. inhibens and proved its crucial role in biofilm formation. In the Roseobacter group, two-thirds of the predicted biofilm plasmids represent the RepA-I type and their physiological role was experimentally validated via plasmid curing for four additional strains. Horizontal transfer of these replicons was documented by a comparison of the RepA-I phylogeny with the species tree. A gene content analysis of 35 RepA-I plasmids revealed a core set of genes, including the rhamnose operon and a specific ABC transporter for polysaccharide export. Taken together, our data show that RepA-I-type biofilm plasmids are essential for the sessile mode of life in the majority of cultivated roseobacters.
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