ArticleInfection and immunity2016
Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Myo5B plays a significant role in the hyphal growth and virulence of the human pathogenic fungusMicrobiology (Reading, England) · 2024Article
- Characterization ofJournal of medical microbiology · 2024Article
- Divergence of cytokinesis and dimorphism control by myosin II regulatory light chain in fission yeasts.iScience · 2023Article
- Transcriptional Profiles of a Foliar Fungal Endophyte (mSystems · 2022Article
- Myosin-II proteins are involved in the growth, morphogenesis, and virulence of the human pathogenic fungusFrontiers in cellular and infection microbiology · 2022Article
- Review
- Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae.Applied microbiology and biotechnology · 2021Article
- The class V myosin interactome of the human pathogen Aspergillus fumigatus reveals novel interactions with COPII vesicle transport proteins.Biochemical and biophysical research communications · 2020Article
- Molecular Mechanisms of Conidial Germination inMicrobiology and molecular biology reviews : MMBR · 2020Review
- Menacing Mold: Recent Advances in Aspergillus Pathogenesis and Host Defense.Journal of molecular biology · 2019Review
- Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence.Scientific reports · 2018Article
- Role of actin depolymerizing factor cofilin in Aspergillus fumigatus oxidative stress response and pathogenesis.Current genetics · 2018Article
- Animal Models of Aspergillosis.Comparative medicine · 2018Review
- Disruption of actin motor function due to MoMyo5 mutation impairs host penetration and pathogenicity in Magnaporthe oryzae.Molecular plant pathology · 2018Article
- The tail domain of theJournal of cell science · 2018Article
- Role of Downregulation and Phosphorylation of Cofilin in Polarized Growth, MpkA Activation and Stress Response ofFrontiers in microbiology · 2018Article
- Molecular Insights Into Development and Virulence Determinants ofFrontiers in cellular and infection microbiology · 2018Review
- Of mice and men and larvae: Galleria mellonella to model the early host-pathogen interactions after fungal infection.Virulence · 2018Article
- Identification of membrane proteome ofFuture science OA · 2017Article
- Proteome Profile of Aspergillus terreus Conidia at Germinating Stage: Identification of Probable Virulent Factors and Enzymes from Mycotoxin Pathways.Mycopathologia · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Myosins are a family of actin-based motor proteins found in many organisms and are categorized into classes based on their structures. Class II and V myosins are known to be important for critical cellular processes, including cytokinesis, endocytosis, exocytosis, and organelle trafficking, in the model fungi Saccharomyces cerevisiae and Aspergillus nidulans However, the roles of myosins in the growth and virulence of the pathogen Aspergillus fumigatus are unknown. We constructed single- and double-deletion strains of the class II and class V myosins in A. fumigatus and found that while the class II myosin (myoB) is dispensable for growth, the class V myosin (myoE) is required for proper hyphal extension; deletion of myoE resulted in hyperbranching and loss of hyphal polarity. Both myoB and myoE are necessary for proper septation, conidiation, and conidial germination, but only myoB is required for conidial viability. Infection with the ΔmyoE strain in the invertebrate Galleria mellonella model and also in a persistently immunosuppressed murine model of invasive aspergillosis resulted in hypovirulence, while analysis of bronchoalveolar lavage fluid revealed that tumor necrosis factor alpha (TNF-α) release and cellular infiltration were similar compared to those of the wild-type strain. The ΔmyoE strain showed fungal growth in the murine lung, while the ΔmyoB strain exhibited little fungal burden, most likely due to the reduced conidial viability. These results show, for the first time, the important role these cytoskeletal components play in the growth of and disease caused by a known pathogen, prompting future studies to understand their regulation and potential targeting for novel antifungal therapies.
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