Evidence map›Paper›PMID 26952870›Full record

ArticleCell stem cell2016

Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth.

Hengli Tang, Christy Hammack, Sarah C Ogden, Zhexing Wen, Xuyu Qian, Yujing Li, Bing Yao, Jaehoon Shin, Feiran Zhang, Emily M Lee and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 744 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
744citing papers in PubMed, 2 pooled it
218.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

744 citing papers in PubMed, 2 syntheses or guidelines pooled it, 1,304 citations in OpenAlex.

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684 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Hengli TangDepartment of Biological Science, Florida State University, Tallahassee, FL 32306, USA. Electronic address: tang@bio.fsu.edu.
Christy HammackDepartment of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Sarah C OgdenDepartment of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Zhexing WenInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA.
Xuyu QianInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA.
Yujing LiDepartment of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Bing YaoDepartment of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Jaehoon ShinInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA.
Feiran ZhangDepartment of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Emily M LeeDepartment of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Kimberly M ChristianInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA.
Ruth A DidierCollege of Medicine, Florida State University, Tallahassee, FL 32306, USA.
Peng JinDepartment of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Hongjun SongInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Solomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA. Electronic address: shongju1@jhmi.edu.
Guo-Li MingInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Solomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21025, USA. Electronic address: gming1@jhmi.edu.
Johns Hopkins University · USFlorida State University · USEmory University · US

Funding

Development of new neurons in the adult brainR01NS047344 · NINDS · JOHNS HOPKINS UNIVERSITY · PI SONG, HONGJUN · 2003 to 2011
$3.4M
Calcium signaling in neuronal navigationR01NS048271 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MING, GUO-LI · 2004 to 2014
$3.2M
Local circuitry mechanisms regulating adult hippocampal neurogenesisR37NS047344 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI SONG, HONGJUN · 2013 to 2019
$2.9M
Tet-mediated Epigenetic Modulation in AutismR01MH102690 · NIMH · EMORY UNIVERSITY · PI JIN, PENG, WARREN, STEPHEN T. · 2014 to 2017
$2.6M
Dissecting Dengue Virus Permissiveness using a Stem Cell Differentiation SystemR21AI119530 · NIAID · FLORIDA STATE UNIVERSITY · PI TANG, HENGLI · 2015 to 2016
$405k
Circuitry mechanisms regulating adult hippocampal neurogenesisR56NS047344 · NINDS · JOHNS HOPKINS UNIVERSITY · PI SONG, HONGJUN · 2012 to 2012
$405k
Function of Lipid Droplets in Viral Entry and Membrane FusionR21AI111250 · NIAID · FLORIDA STATE UNIVERSITY · PI TANG, HENGLI · 2014 to 2015
$402k
Development of forebrain organoid platform for modelling human cortical neurogenesisR21NS095348 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MING, GUO-LI · 2015 to 2016
$294k
NIAID NIH HHS R21 AI111250NIAID NIH HHS R21 AI119530NIMH NIH HHS R01 MH102690NINDS NIH HHS R01 NS047344NINDS NIH HHS R01 NS048271NINDS NIH HHS R21 NS095348NINDS NIH HHS R37 NS047344NINDS NIH HHS R56 NS047344
6 · The paper itself

Abstract

The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficiently infects human neural progenitor cells (hNPCs) derived from induced pluripotent stem cells. Infected hNPCs further release infectious ZIKV particles. Importantly, ZIKV infection increases cell death and dysregulates cell-cycle progression, resulting in attenuated hNPC growth. Global gene expression analysis of infected hNPCs reveals transcriptional dysregulation, notably of cell-cycle-related pathways. Our results identify hNPCs as a direct ZIKV target. In addition, we establish a tractable experimental model system to investigate the impact and mechanism of ZIKV on human brain development and provide a platform to screen therapeutic compounds.

Indexed as

Cell CycleCell DeathCell ProliferationGene Expression RegulationHumansInduced Pluripotent Stem CellsNeural Stem CellsZika VirusZika Virus Infection

Identifiers

PMID26952870
PMCPMC5299540
OpenAlexW2292422784

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.