ArticleJournal of biomedical science2016
Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci.
Article in Journal of biomedical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Proportion of toxin and non-toxin virulence factors of Staphylococcus aureus isolates from diabetic foot infection: a systematic review and meta-analysis.BMC microbiology · 2024Pooled it
- Use of TaqMan array card to investigate bacterial isolates in diabetic foot ulcers.Journal of medical microbiology · 2026Article
- Risk Factors Associated With Methicillin-ResistantThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026Article
- Clinical severity-dependent virulence ofFrontiers in cellular and infection microbiology · 2026Article
- In Vivo Antimicrobial Activity of Nisin Z AgainstAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Absence of Synergism between a Dual-AMP Biogel and Antibiotics Used as Therapeutic Agents for Diabetic Foot Infections.International journal of molecular sciences · 2023Article
- Whole genome sequencing and pan-genome analysis of Staphylococcus/Mammaliicoccus spp. isolated from diabetic foot ulcers and contralateral healthy skin of Algerian patients.BMC microbiology · 2023Article
- Quantitative Imaging of the Action of vCPP2319, an Antimicrobial Peptide from a Viral Scaffold, againstACS infectious diseases · 2023Article
- Review
- Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- The Wound Microbiome.Cold Spring Harbor perspectives in biology · 2023Review
- An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm ofPharmaceuticals (Basel, Switzerland) · 2023Article
- Bacteriostatic and Antibiofilm Efficacy of a Nisin Z Solution against Co-Cultures ofLife (Basel, Switzerland) · 2023Article
- Antibacterial Activity of Surfactin and Synergistic Effect with Conventional Antibiotics Against Methicillin-ResistantDiabetes, metabolic syndrome and obesity : targets and therapy · 2023Article
- Clinical and Bacteriological Analyses of Biofilm-Forming Staphylococci Isolated from Diabetic Foot Ulcers.Infection and drug resistance · 2023Article
- Review
- MRSA in Humans, Pets and Livestock in Portugal: Where We Came from and Where We Are Going.Pathogens (Basel, Switzerland) · 2022Review
- Nisin Mutant Prevention Concentration and the Role of Subinhibitory Concentrations on Resistance Development by Diabetic FootAntibiotics (Basel, Switzerland) · 2022Article
- Strategies to prevent, curb and eliminate biofilm formation based on the characteristics of various periods in one biofilm life cycle.Frontiers in cellular and infection microbiology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDiabetes mellitus is a major chronic disease that continues to increase significantly. One of the most important and costly complications of diabetes are foot infections that may be colonized by pathogenic and antimicrobial resistant bacteria, harboring several virulence factors, that could impair its successful treatment. Staphylococcus aureus is one of the most prevalent isolate in diabetic foot infections, together with aerobes and anaerobes.
methodsIn this study, conducted in the Lisbon area, staphylococci isolated (n = 53) from diabetic foot ulcers were identified, genotyped and screened for virulence and antimicrobial resistance traits. Genetic relationship amongst isolates was evaluated by pulsed-field-gel-electrophoresis with further multilocus sequence typing of the identified pulsotypes. PCR was applied for detection of 12 virulence genes and e-test technique was performed to determine minimal inhibitory concentration of ten antibiotics.
resultsAmong the 53 isolates included in this study, 41 Staphylococcus aureus were identified. Staphylococcal isolates were positive for intercellular adhesins icaA and icaD, negative for biofilm associated protein bap and pantone-valentine leucocidin pvl. S. aureus quorum sensing genes agrI and agrII were identified and only one isolate was positive for toxic shock syndrome toxin tst. 36 % of staphylococci tested were multiresistant and higher rates of resistance were obtained for ciprofloxacin and erythromycin. Clonality analysis revealed high genomic diversity and numerous S. aureus sequence types, both community- and hospital-acquired, belonging mostly to clonal complexes CC5 and C22, widely diffused in Portugal nowadays.
conclusionsThis study shows that diabetic foot ulcer staphylococci are genomically diverse, present resistance to medically important antibiotics and harbour virulence determinants. These properties suggest staphylococci can contribute to persistence and severity of these infections, leading to treatment failure and to the possibility of transmitting these features to other microorganisms sharing the same niche. In this context, diabetic patients may become a transmission vehicle for microorganisms' clones between community and clinical environments.
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