ArticleThe Korean journal of parasitology2016
Isolation and Genotyping of Toxoplasma gondii Strains in Ovine Aborted Fetuses in Khorasan Razavi Province, Iran.
Article in The Korean journal of parasitology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Unraveling the link: serological and molecular insights into Toxoplasma gondii infection in women with spontaneous abortion history.Tropical diseases, travel medicine and vaccines · 2025Article
- Serological and molecular survey of Toxoplasma Gondii in aborted livestock fetuses from Northeast Iran.BMC research notes · 2024Article
- Molecular Detection ofIranian journal of public health · 2024Article
- Is GRA6 Gene a Suitable Marker for Molecular Typing of Toxoplasma Gondii? A Scoping Systematic Review.Acta parasitologica · 2024Article
- Molecular prevalence and genetic diversity ofOpen veterinary journal · 2023Article
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- Molecular and serological detection and ofJournal fur Verbraucherschutz und Lebensmittelsicherheit = Journal of consumer protection and food safety · 2021Article
- Review
- Prevalence and molecular characterization ofFood and waterborne parasitology · 2018Article
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- Molecular and Pathological Detection ofIranian journal of parasitologyArticle
- The First Survey of Isolation and Molecular Typing ofIranian journal of parasitologyArticle
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- Molecular Genotyping ofIranian journal of parasitologyArticle
- More Than Seventy Years of Research (1948-November 2021) onIranian journal of parasitologyReview
- Genetic Diversity ofIranian journal of parasitologyArticle
- Serological Evaluation and Molecular Genotyping ofIranian journal of parasitologyArticle
- Molecular Detection and Genotypic Characterization ofInternational journal of fertility & sterilityArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxoplasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by nested PCR. The positive brain samples were bioassayed in Webster Swiss mice. The serum samples of mice were examined for T. gondii antibodies by IFAT at 6 weeks post inoculation, and T. gondii cysts were searched in brain tissue samples of seropositive mice. The positive samples were genotyped by using a PCR-RLFP method. Subsequently, GRA6 sequences of isolates were analyzed using a phylogenetic method. The results revealed that T. gondii DNA was detected in 54% (20/37, 95% CI 38.4-69.0%) brain samples of ovine aborted fetuses. In bioassay of mice, only 2 samples were virulent and the mice were killed at 30 days post inoculation, while the others were non-virulent to mice. The size of cysts ranged 7-22 µm. Complete genotyping data for GRA6 locus were observed in 5 of the 20 samples. PCR-RLFP results and phylogenetic analysis revealed that all of the isolated samples were closely related to type I. For the first time, we could genotype and report T. gondii isolates from ovine aborted fetuses in Khorasan Razavi Province, Iran. The results indicate that the T. gondii isolates are genetically related to type I, although most of them were non-virulent for mice.
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