ArticleProceedings of the National Academy of Sciences of the United States of America2016
Three-dimensional bioprinting of thick vascularized tissues.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 599 papers.
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Who cites it
599 citing papers in PubMed.
- Engineering high-fidelity bone organoids: Operational classification, multilineage crosstalk, biofabrication evidence, and translational validation.Materials today. Bio · 2026Article
- Suspended particles for omnidirectional template sacrifice for rapid vascular patterning within engineered tissues.Science advances · 2026Article
- A one-step volumetric biofabrication platform for complex hydrogel-based hollow biomaterials.Materials today. Bio · 2026Article
- Three-Dimensional Bioprinting in Reconstructive Plastic Surgery: A Comprehensive Review.Cells · 2026Review
- Vascularized Brain Organoids: Advances in Engineering Human Neurovascular Models.International journal of stem cells · 2026Review
- Hydrogels: current biomedical applications and future directions.Molecular biomedicine · 2026Review
- Coconut Oil as Lipid-Based 3D-Printable Sacrificial Material for the Facile Biofabrication of Precise Perfusable Microchannels and Freeform Hydrogel Structures.Small science · 2026Article
- Integrating microchannels and flows into 3D printable granular hydrogel matrices.Lab on a chip · 2026Article
- Topological Tracks Patterned via 3D Printing Vascularize Murine Organ-Scale Constructs.Cell biomaterials · 2026Article
- Accessible Biofabrication of Anatomically Inspired Hollow and Branched Hydrogel Constructs by Soft Templating (Sof-T).Bioengineering (Basel, Switzerland) · 2026Article
- 4D force patterning enables spatial control of angiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Support-Enabled 3D Printing of Complex Anisotropic Hydrogel Structures Using Cellulose-Based Inks: Pathways Toward Biomimetic Human Aorta Models.ACS biomaterials science & engineering · 2026Article
- Photothermal Ablation Blotting for Capillary-Scale Microvasculature Engineering.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Advancing Stem Cell Bioprinting to Bridge Engineered and Natural Tissue Constructs.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Spontaneous Helical Alignment of Smooth Muscle Cells to Form a Medial Layer for Engineered Microvasculature.Advanced healthcare materials · 2026Article
- Biopolymer-Based 3D Printing for Dental-Pulp Complex Tissue Regeneration: Innovations and Challenges.Molecules (Basel, Switzerland) · 2026Review
- A Roadmap to Perfused Skin: Defining the Next Generation of Research Questions in Cutaneous Tissue Engineering.International journal of molecular sciences · 2026Review
- Spatial distribution and density of fibroblasts determine angiogenic response of endothelial cells.Research square · 2026Article
- Effect of 3D-printed co-culture design of mesenchymal stem cells and human umbilical vein endothelial cells on tubular formation.Scientific reports · 2026Article
- Kidney organoid vascularization: current advancements in the field.Regenerative therapy · 2026Article
539 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin tissues that only survive for short durations. To improve their physiological relevance, we report a method for bioprinting 3D cell-laden, vascularized tissues that exceed 1 cm in thickness and can be perfused on chip for long time periods (>6 wk). Specifically, we integrate parenchyma, stroma, and endothelium into a single thick tissue by coprinting multiple inks composed of human mesenchymal stem cells (hMSCs) and human neonatal dermal fibroblasts (hNDFs) within a customized extracellular matrix alongside embedded vasculature, which is subsequently lined with human umbilical vein endothelial cells (HUVECs). These thick vascularized tissues are actively perfused with growth factors to differentiate hMSCs toward an osteogenic lineage in situ. This longitudinal study of emergent biological phenomena in complex microenvironments represents a foundational step in human tissue generation.
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