ArticlePlant physiology2016
Asparagus IRX9, IRX10, and IRX14A Are Components of an Active Xylan Backbone Synthase Complex that Forms in the Golgi Apparatus.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 114 citations in OpenAlex.
- Revealing the genetic regulation of wood traits and secondary cell wall development inHorticulture research · 2026Article
- Overexpression of a Xylem-Dominant Expressing BTB Gene,Plants (Basel, Switzerland) · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- Enzymatic characterization and docking simulation of a xylan synthase catalytic subunit,Plant biotechnology (Tokyo, Japan) · 2025Article
- Multiprotein Complexes of Plant Glycosyltransferases Involved in Their Function and Trafficking.Plants (Basel, Switzerland) · 2025Review
- XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice.The Plant cell · 2024Article
- Review
- The structure and interaction of polymers affects secondary cell wall banding patterns in Arabidopsis.The Plant cell · 2024Article
- Identification of glycosyltransferases mediating 2-O-arabinopyranosyl and 2-O-galactosyl substitutions of glucuronosyl side chains of xylan.The Plant journal : for cell and molecular biology · 2024Article
- Mining Candidate Genes for Leaf Angle inInternational journal of molecular sciences · 2024Article
- Identification and Functional Verification of the Glycosyltransferase Gene Family Involved in Flavonoid Synthesis inPlants (Basel, Switzerland) · 2024Article
- Cytochemical localization and synthesis mechanism of the glucomannan in pseudobulbs ofHorticulture research · 2024Article
- A comprehensive review on genetic modification of plant cell wall for improved saccharification efficiency.Molecular biology reports · 2023Review
- An update on xylan structure, biosynthesis, and potential commercial applications.Cell surface (Amsterdam, Netherlands) · 2023Review
- Genetic Approaches to Increase Arabinoxylan and β-Glucan Content in Wheat.Plants (Basel, Switzerland) · 2023Review
- Impact of xylan on field productivity and wood saccharification properties in aspen.Frontiers in plant science · 2023Article
- Promoter and domain structures regulate FLA12 function during Arabidopsis secondary wall development.Frontiers in plant science · 2023Article
- Xylan-based nanocompartments orchestrate plant vessel wall patterning.Nature plants · 2022Article
- Critical Determinants in ER-Golgi Trafficking of Enzymes Involved in Glycosylation.Plants (Basel, Switzerland) · 2022Review
- Modification of plant cell walls with hydroxycinnamic acids by BAHD acyltransferases.Frontiers in plant science · 2022Review
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heteroxylans are abundant components of plant cell walls and provide important raw materials for the food, pharmaceutical, and biofuel industries. A number of studies in Arabidopsis (Arabidopsis thaliana) have suggested that the IRREGULAR XYLEM9 (IRX9), IRX10, and IRX14 proteins, as well as their homologs, are involved in xylan synthesis via a Golgi-localized complex termed the xylan synthase complex (XSC). However, both the biochemical and cell biological research lags the genetic and molecular evidence. In this study, we characterized garden asparagus (Asparagus officinalis) stem xylan biosynthesis genes (AoIRX9, AoIRX9L, AoIRX10, AoIRX14A, and AoIRX14B) by heterologous expression in Nicotiana benthamiana We reconstituted and partially purified an active XSC and showed that three proteins, AoIRX9, AoIRX10, and AoIRX14A, are necessary for xylan xylosyltranferase activity in planta. To better understand the XSC structure and its composition, we carried out coimmunoprecipitation and bimolecular fluorescence complementation analysis to show the molecular interactions between these three IRX proteins. Using a site-directed mutagenesis approach, we showed that the DxD motifs of AoIRX10 and AoIRX14A are crucial for the catalytic activity. These data provide, to our knowledge, the first lines of biochemical and cell biological evidence that AoIRX9, AoIRX10, and AoIRX14A are core components of a Golgi-localized XSC, each with distinct roles for effective heteroxylan biosynthesis.
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