ArticlePlant physiology2016
The Chloroplastic Protein THF1 Interacts with the Coiled-Coil Domain of the Disease Resistance Protein N' and Regulates Light-Dependent Cell Death.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- The barley disease resistance proteinmicroPublication biology · 2026Article
- Genetic Mapping and Transcriptomic Analysis of Sepal-Derived Seed Shape in Spinach.International journal of molecular sciences · 2025Article
- Co-occurrence of chloroplastic ROS production and salicylic acid induction in plant immunity.The New phytologist · 2025Article
- Unveiling the Role of RNA Recognition Motif Proteins in Orchestrating Nucleotide-Binding Site and Leucine-Rich Repeat Protein Gene Pairs and Chloroplast Immunity Pathways: Insights into Plant Defense Mechanisms.International journal of molecular sciences · 2024Article
- Alternaria TeA toxin activates a chloroplast retrograde signaling pathway to facilitate JA-dependent pathogenicity.Plant communications · 2024Article
- Near-infrared light and PIF4 promote plant antiviral defense by enhancing RNA interference.Plant communications · 2024Article
- Two functional CC-NBS-LRR proteins from rye chromosome 6RS confer differential age-related powdery mildew resistance to wheat.Plant biotechnology journal · 2024Article
- Unraveling the Mechanisms of Virus-Induced Symptom Development in Plants.Plants (Basel, Switzerland) · 2023Review
- Tuning the Wavelength: Manipulation of Light Signaling to Control Plant Defense.International journal of molecular sciences · 2023Review
- Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data.Methods in molecular biology (Clifton, N.J.) · 2023Article
- N and N'-mediated recognition confers resistance to tomato brown rugose fruit virus.microPublication biology · 2022Article
- Plasma membrane-localized plant immune receptor targets HThe New phytologist · 2022Article
- Function of Chloroplasts in Plant Stress Responses.International journal of molecular sciences · 2021Review
- Maize nicotinate N-methyltransferase interacts with the NLR protein Rp1-D21 and modulates the hypersensitive response.Molecular plant pathology · 2021Article
- Genome-wide functional analysis of hot pepper immune receptors reveals an autonomous NLR clade in seed plants.The New phytologist · 2021Article
- Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance.Molecular plant pathology · 2020Article
- Impaired Expression of Chloroplast HSP90C Chaperone Activates Plant Defense Responses with a Possible Link to a Disease-Symptom-Like Phenotype.International journal of molecular sciences · 2020Article
- Dissection of Cell Death Induction by Wheat Stem Rust Resistance Protein Sr35 and Its Matching Effector AvrSr35.Molecular plant-microbe interactions : MPMI · 2020Article
- The rice NLR pair Pikp-1/Pikp-2 initiates cell death through receptor cooperation rather than negative regulation.PloS one · 2020Article
- Uncoiling CNLs: Structure/Function Approaches to Understanding CC Domain Function in Plant NLRs.Plant & cell physiology · 2018Review
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One branch of plant immunity is mediated through nucleotide-binding/Leu-rich repeat (NB-LRR) family proteins that recognize specific effectors encoded by pathogens. Members of the I2-like family constitute a well-conserved subgroup of NB-LRRs from Solanaceae possessing a coiled-coil (CC) domain at their N termini. We show here that the CC domains of several I2-like proteins are able to induce a hypersensitive response (HR), a form of programmed cell death associated with disease resistance. Using yeast two-hybrid screens, we identified the chloroplastic protein Thylakoid Formation1 (THF1) as an interacting partner for several I2-like CC domains. Co-immunoprecipitations and bimolecular fluorescence complementation assays confirmed that THF1 and I2-like CC domains interact in planta and that these interactions take place in the cytosol. Several HR-inducing I2-like CC domains have a negative effect on the accumulation of THF1, suggesting that the latter is destabilized by active CC domains. To confirm this model, we investigated N', which recognizes the coat protein of most Tobamoviruses, as a prototypical member of the I2-like family. Transient expression and gene silencing data indicated that THF1 functions as a negative regulator of cell death and that activation of full-length N' results in the destabilization of THF1. Consistent with the known function of THF1 in maintaining chloroplast homeostasis, we show that the HR induced by N' is light-dependent. Together, our results define, to our knowledge, novel molecular mechanisms linking light and chloroplasts to the induction of cell death by a subgroup of NB-LRR proteins.
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