ArticleNucleic acids research2016
In depth analysis of the mechanism of action of metal-dependent sigma factors: characterization of CorE2 from Myxococcus xanthus.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Current models in bacterial hemicellulase-encoding gene regulation.Applied microbiology and biotechnology · 2024Review
- Siderophores and competition for iron govern myxobacterial predation dynamics.The ISME journal · 2024Article
- The Burkholderia cenocepacia iron starvation σ factor, OrbS, possesses an on-board iron sensor.Nucleic acids research · 2022Article
- Mechanisms of Action of Non-Canonical ECF Sigma Factors.International journal of molecular sciences · 2022Review
- Antimicrobial Activity of Metals and Metalloids.Annual review of microbiology · 2021Review
- The bacterial iron sensor IdeR recognizes its DNA targets by indirect readout.Nucleic acids research · 2021Article
- Expansion and re-classification of the extracytoplasmic function (ECF) σ factor family.Nucleic acids research · 2021Article
- The novel ECF56 SigG1-RsfG system modulates morphological differentiation and metal-ion homeostasis in Streptomyces tsukubaensis.Scientific reports · 2020Article
- Evolution of the extracytoplasmic function σ factor protein family.NAR genomics and bioinformatics · 2020Article
- Copper and Melanin Play a Role inFrontiers in microbiology · 2020Article
- Metal-responsive RNA polymerase extracytoplasmic function (ECF) sigma factors.Molecular microbiology · 2019Review
- Evolutionary couplings of amino acid residues reveal structure and function of bacterial signaling proteins.Molecular microbiology · 2019Article
- Long-term soil metal exposure impaired temporal variation in microbial metatranscriptomes and enriched active phages.Microbiome · 2018Article
- Characterization of the Widely Distributed Novel ECF42 Group of Extracytoplasmic Function σ Factors in Streptomyces venezuelae.Journal of bacteriology · 2018Article
- The fused SnoaL_2 domain in the Mycobacterium tuberculosis sigma factor σJ modulates promoter recognition.Nucleic acids research · 2017Article
- Myxobacteria: Moving, Killing, Feeding, and Surviving Together.Frontiers in microbiology · 2016Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracytoplasmic function sigma factors represent the third pillar of signal-transduction mechanisms in bacteria. The variety of stimuli they recognize and mechanisms of action they use have allowed their classification into more than 50 groups. We have characterized CorE2 from Myxococcus xanthus, which belongs to group ECF44 and upregulates the expression of two genes when it is activated by cadmium and zinc. Sigma factors of this group contain a Cys-rich domain (CRD) at the C terminus which is essential for detecting metals. Point mutations at the six Cys residues of the CRD have revealed the contribution of each residue to CorE2 activity. Some of them are essential, while others are either dispensable or their mutations only slightly affect the activity of the protein. However, importantly, mutation of Cys174 completely shifts the specificity of CorE2 from cadmium to copper, indicating that the Cys arrangement of the CRD determines the metal specificity. Moreover, the conserved CxC motif located between the σ2 domain and the σ4.2 region has also been found to be essential for activity. The results presented here contribute to our understanding of the mechanism of action of metal-dependent sigma factors and help to define new common features of the members of this group of regulators.
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