Evidence map›Paper›PMID 26951114›Full record

ArticleMammalian genome : official journal of the International Mammalian Genome Society2016

Murine diet/tissue and human brain tumorigenesis alter Mthfr/MTHFR 5'-end methylation.

Nancy Lévesque, Daniel Leclerc, Tenzin Gayden, Anthoula Lazaris, Nicolas De Jay, Stephanie Petrillo, Peter Metrakos, Nada Jabado, Rima Rozen

Abstract read
PubMed Publisher
In one paragraph

Article in Mammalian genome : official journal of the International Mammalian Genome Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Epigenetics of Male Infertility: The Role of DNA Methylation.Frontiers in cell and developmental biology · 2021
    Review
  2. Dietary Modulation of the Epigenome.Physiological reviews · 2018
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Nancy LévesqueDepartments of Human Genetics and Pediatrics, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Bloc E, Rm E M0.3211, Montreal, H4A 3J1, Canada.
Daniel LeclercDepartments of Human Genetics and Pediatrics, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Bloc E, Rm E M0.3211, Montreal, H4A 3J1, Canada.
Tenzin GaydenDivision of Experimental Medicine, Departments of Pediatrics and Human Genetics, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Montreal, H4A 3J1, Canada.
Anthoula LazarisDepartment of Surgery, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Montreal, H4A 3J1, Canada.
Nicolas De JayDivision of Experimental Medicine, Departments of Pediatrics and Human Genetics, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Montreal, H4A 3J1, Canada.
Stephanie PetrilloDepartment of Surgery, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Montreal, H4A 3J1, Canada.
Peter MetrakosDepartment of Surgery, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Montreal, H4A 3J1, Canada.
Nada JabadoDivision of Experimental Medicine, Departments of Pediatrics and Human Genetics, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Montreal, H4A 3J1, Canada.
Rima RozenDepartments of Human Genetics and Pediatrics, The Research Institute of the McGill University Health Centre, McGill University, 1001 Décarie Blvd., Bloc E, Rm E M0.3211, Montreal, H4A 3J1, Canada. rima.rozen@mcgill.ca.
McGill University Health Centre · CA

Funding

Canadian Institutes of Health Research MOP 43232
6 · The paper itself

Abstract

Polymorphisms and decreased activity of methylenetetrahydrofolate reductase (MTHFR) are linked to disease, including cancer. However, epigenetic regulation has not been thoroughly studied. Our goal was to generate DNA methylation profiles of murine/human MTHFR gene regions and examine methylation in brain and liver tumors. Pyrosequencing in four murine tissues revealed minimal DNA methylation in the CpG island. Higher methylation was seen in liver or intestine in the CpG island shore 5' to the upstream translational start site or in another region 3' to the downstream start site. In the latter region, there was negative correlation between expression and methylation. Three orthologous regions were investigated in human MTHFR, as well as a fourth region between the two translation start sites. We found significantly increased methylation in three regions (not the CpG island) in pediatric astrocytomas compared with control brain, with decreased expression in tumors. Methylation in hepatic carcinomas was also increased in the three regions compared with normal liver, but the difference was significant for only one CpG. This work, the first overview of the Mthfr/MTHFR epigenetic landscape, suggests regulation through methylation in some regions, demonstrates increased methylation/decreased expression in pediatric astrocytomas, and should serve as a resource for future epigenetic studies.

Indexed as

Cell Transformation, NeoplasticDietDNA MethylationAnimalsBase SequenceBrainBrain NeoplasmsCpG IslandsDisease Models, AnimalEpigenesis, GeneticFemaleGene ExpressionGenetic LociHumansLiver NeoplasmsMethylenetetrahydrofolate Reductase (NADPH2)Methylenetetrahydrofolate Reductase (NADPH2)

Identifiers

PMID26951114
OpenAlexW2296655555

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.