ArticleAsian-Australasian journal of animal sciences2016
Characterizing Milk Production Related Genes in Holstein Using RNA-seq.
Article in Asian-Australasian journal of animal sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Genome-Wide Gene-Environment Interaction Analysis Identifies Novel Candidate Variants for Growth Traits in Beef Cattle.Animals : an open access journal from MDPI · 2024Article
- Genetic legacy and adaptive signatures: investigating the history, diversity, and selection signatures in Rendena cattle resilient to eighteenth century rinderpest epidemics.Genetics, selection, evolution : GSE · 2024Article
- Genomic selection pressure discovery using site-frequency spectrum and reduced local variability statistics in Pakistani Dera-Din-Panah goat.Tropical animal health and production · 2023Article
- Transcriptome-wide mapping of milk somatic cells upon subclinical mastitis infection in dairy cattle.Journal of animal science and biotechnology · 2023Article
- Multi-Omics Integration and Network Analysis Reveal Potential Hub Genes and Genetic Mechanisms Regulating Bovine Mastitis.Current issues in molecular biology · 2022Article
- Lactation Associated Genes Revealed in Holstein Dairy Cows by Weighted Gene Co-Expression Network Analysis (WGCNA).Animals : an open access journal from MDPI · 2021Article
- Overview of Folic Acid Supplementation Alone or in Combination with Vitamin B12 in Dairy Cattle during Periparturient Period.Metabolites · 2020Review
- Article
- Comparison of the Transcriptome of the Ovine Mammary Gland in Lactating and Non-lactating Small-Tailed Han Sheep.Frontiers in genetics · 2020Article
- Identification of novel molecular markers of mastitis caused byJournal of animal science and biotechnology · 2020Article
- Whole blood transcriptome analysis reveals footprints of cattle adaptation to sub-arctic conditions.Animal genetics · 2019Article
- Identifying Hub Genes for Heat Tolerance in Water Buffalo (Frontiers in genetics · 2019Article
- Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing.PloS one · 2018Article
- RNA-seq analysis for detecting quantitative trait-associated genes.Scientific reports · 2016Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. RNA-seq data was collected from 21 Holstein samples, along with group information about milk production ability; milk yield; and protein, fat, and solid contents. Meta-analysis was employed in order to generally characterize genes related to milk production. In addition, we attempted to investigate the relationship between milk related traits, parity, and lactation period. We observed that milk fat is highly correlated with lactation period; this result indicates that this effect should be considered in the model in order to accurately detect milk production related genes. By employing our developed model, 271 genes were significantly (false discovery rate [FDR] adjusted p-value<0.1) detected as milk production related differentially expressed genes. Of these genes, five (albumin, nitric oxide synthase 3, RNA-binding region (RNP1, RRM) containing 3, secreted and transmembrane 1, and serine palmitoyltransferase, small subunit B) were technically validated using quantitative real-time polymerase chain reaction (qRT-PCR) in order to check the accuracy of RNA-seq analysis. Finally, 83 gene ontology biological processes including several blood vessel and mammary gland development related terms, were significantly detected using DAVID gene-set enrichment analysis. From these results, we observed that detected milk production related genes are highly enriched in the circulation system process and mammary gland related biological functions. In addition, we observed that detected genes including caveolin 1, mammary serum amyloid A3.2, lingual antimicrobial peptide, cathelicidin 4 (CATHL4), cathelicidin 6 (CATHL6) have been reported in other species as milk production related gene. For this reason, we concluded that our detected 271 genes would be strong candidates for determining milk production.
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