ArticleMolecular plant pathology2017
Optimization of the HyPer sensor for robust real-time detection of hydrogen peroxide in the rice blast fungus.
Article in Molecular plant pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Recent advances in the efficient degradation of lignocellulosic metabolic networks by lytic polysaccharide monooxygenase.Acta biochimica et biophysica Sinica · 2023Review
- Heme sensing and trafficking in fungi.Fungal biology reviews · 2023Article
- Reactive oxygen species and nitric oxide as mediators in plant hypersensitive response and stomatal closure.Plant signaling & behavior · 2021Article
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Every Coin Has Two Sides: Reactive Oxygen Species during Rice⁻International journal of molecular sciences · 2019Review
- Insights into the HyPer biosensor as molecular tool for monitoring cellular antioxidant capacity.Redox biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) production and breakdown have been studied in detail in plant-pathogenic fungi, including the rice blast fungus, Magnaporthe oryzae; however, the examination of the dynamic process of ROS production in real time has proven to be challenging. We resynthesized an existing ROS sensor, called HyPer, to exhibit optimized codon bias for fungi, specifically Neurospora crassa, and used a combination of microscopy and plate reader assays to determine whether this construct could detect changes in fungal ROS during the plant infection process. Using confocal microscopy, we were able to visualize fluctuating ROS levels during the formation of an appressorium on an artificial hydrophobic surface, as well as during infection on host leaves. Using the plate reader, we were able to ascertain measurements of hydrogen peroxide (H
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Registered trials
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