ArticlePloS one2016
Extremely Low-Frequency Electromagnetic Fields Promote In Vitro Neuronal Differentiation and Neurite Outgrowth of Embryonic Neural Stem Cells via Up-Regulating TRPC1.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Mapping the Landscape of Magnetic Field Effects on Neural Regeneration and Repair: A Combined Systematic Review, Mathematical Model, and Meta-Analysis.Journal of tissue engineering and regenerative medicine · 2023Pooled it
- Trial
- The consequences of the extremely low frequency electromagnetic field (ELF-EMF) exposure on physiological processes in the uterus: novel insights and implications.Frontiers in physiology · 2026Review
- Extremely low-frequency electromagnetic fields facilitate proliferation and functional differentiation in spinal neural stem cells.Scientific reports · 2025Article
- Pulsed electromagnetic stimulation promotes neuronal maturation by up-regulating cholesterol biosynthesis.Stem cell research & therapy · 2025Article
- Enhanced neuromorphogenesis of neural stem cells via the optimization of physical stimulus-responsive signaling pathways.Stem cell research & therapy · 2025Article
- Fecal Microbiota Transplantation from Mice Receiving Magnetic Mitohormesis Treatment Reverses High-Fat Diet-Induced Metabolic and Osteogenic Dysfunction.International journal of molecular sciences · 2025Article
- Neurobiological effects and mechanisms of magnetic fields: a review from 2000 to 2023.BMC public health · 2024Review
- Stimuli-responsive microcarriers and their application in tissue repair: A review of magnetic and electroactive microcarrier.Bioactive materials · 2024Review
- Wrapping stem cells with wireless electrical nanopatches for traumatic brain injury therapy.Nature communications · 2024Article
- Review
- Electroceuticals and Magnetoceuticals in Gastroenterology.Biomolecules · 2024Review
- Synergistic Cellular Responses Conferred by Concurrent Optical and Magnetic Stimulation Are Attenuated by Simultaneous Exposure to Streptomycin: An Antibiotic Dilemma.Bioengineering (Basel, Switzerland) · 2024Article
- The Cellular Response Is Determined by a Combination of Different ELF-EMF Exposure Parameters: A Scope Review.International journal of molecular sciences · 2024Review
- Central Causation of Autism/ASDs via Excessive [CaBrain sciences · 2024Review
- Pulsating Extremely Low-Frequency Electromagnetic Fields Influence Differentiation of Mouse Neural Stem Cells towards Astrocyte-like Phenotypes: In Vitro Pilot Study.International journal of molecular sciences · 2024Article
- Harmonizing Magnetic Mitohormetic Regenerative Strategies: Developmental Implications of a Calcium-Mitochondrial Axis Invoked by Magnetic Field Exposure.Bioengineering (Basel, Switzerland) · 2023Review
- The Developmental Implications of Muscle-Targeted Magnetic Mitohormesis: A Human Health and Longevity Perspective.Bioengineering (Basel, Switzerland) · 2023Review
- Associations of solar activity and related exposures with fetal growth.The Science of the total environment · 2023Article
- Article
Corrections and comments
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Authors and funding
19 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) can enhance hippocampal neurogenesis in adult mice. However, little is focused on the effects of ELF-EMFs on embryonic neurogenesis. Here, we studied the potential effects of ELF-EMFs on embryonic neural stem cells (eNSCs). We exposed eNSCs to ELF-EMF (50 Hz, 1 mT) for 1, 2, and 3 days with 4 hours per day. We found that eNSC proliferation and maintenance were significantly enhanced after ELF-EMF exposure in proliferation medium. ELF-EMF exposure increased the ratio of differentiated neurons and promoted the neurite outgrowth of eNSC-derived neurons without influencing astrocyes differentiation and the cell apoptosis. In addition, the expression of the proneural genes, NeuroD and Ngn1, which are crucial for neuronal differentiation and neurite outgrowth, was increased after ELF-EMF exposure. Moreover, the expression of transient receptor potential canonical 1 (TRPC1) was significantly up-regulated accompanied by increased the peak amplitude of intracellular calcium level induced by ELF-EMF. Furthermore, silencing TRPC1 expression eliminated the up-regulation of the proneural genes and the promotion of neuronal differentiation and neurite outgrowth induced by ELF-EMF. These results suggest that ELF-EMF exposure promotes the neuronal differentiation and neurite outgrowth of eNSCs via up-regulation the expression of TRPC1 and proneural genes (NeuroD and Ngn1). These findings also provide new insights in understanding the effects of ELF-EMF exposure on embryonic brain development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.