ArticleApplications in plant sciences2016
Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae).
Article in Applications in plant sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Exclusion of bird pollinators impacts mating system and reduces offspring fitness in a pollination-generalist tree.Annals of botany · 2025Article
- Chromosome-level genome assembly of Elaeocarpus petiolatus (Elaeocarpaceae).Scientific data · 2025Article
- Article
- Unexpectedly low paternal diversity is associated with infrequent pollinator visitation for a bird-pollinated plant.Oecologia · 2021Article
- Isolation and characterization of microsatellite primers for the critically endangered shrubApplications in plant sciences · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
premise of the studyMicrosatellite markers were developed for the rare Tetratheca erubescens (Elaeocarpaceae) to assess genetic diversity and spatial structuring. METHODS AND
resultsWe generated ca. 2.7 million sequence reads using a Personal Genome Machine (PGM) semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80-480 bp. From these, 30 primer pairs were selected and screened using PCR, from which 11 loci were found to be polymorphic and amplified reliably. For a sample of 95 plants from three populations, the number of alleles observed for these 11 loci ranged from two to seven and expected heterozygosity ranged from 0.06 to 0.72. No consistent evidence for null alleles or departure from Hardy-Weinberg equilibrium was found for any of the 11 loci.
conclusionsThese markers will enable the quantification of genetic impact of proposed mining activities on the narrow endemic T. erubescens.
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