Evidence map›Paper›PMID 26949576›Full record

ArticleApplications in plant sciences2016

Isolation and characterization of novel microsatellite loci for the endangered orchid Cypripedium japonicum (Orchidaceae).

Yumi Yamashita, Ayako Izuno, Yuji Isagi, Takahide Kurosawa, Shingo Kaneko

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applications in plant sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Environmental conservation value of an endangered species: the case of Cypripedium Japonicum.Environmental science and pollution research international · 2021
    Article
  4. Development of microsatellite markers forApplications in plant sciences · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yumi YamashitaGraduate School of Symbiotic Systems Science and Technology, Fukushima University, Kanayagawa 1, Fukushima, Fukushima 960-1296, Japan.
Ayako IzunoLaboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Yuji IsagiLaboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Takahide KurosawaGraduate School of Symbiotic Systems Science and Technology, Fukushima University, Kanayagawa 1, Fukushima, Fukushima 960-1296, Japan.
Shingo KanekoGraduate School of Symbiotic Systems Science and Technology, Fukushima University, Kanayagawa 1, Fukushima, Fukushima 960-1296, Japan.
Fukushima University · JPKyoto University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyTwenty-six microsatellite markers were developed for the endangered orchid Cypripedium japonicum (Orchidaceae) to estimate the clonal diversity and genetic structure of the remaining populations in Japan. METHODS AND

resultsMicrosatellite loci of C. japonicum were isolated using Ion Personal Genome Machine (PGM) sequencing. The primer sets were tested on 55 ramets sampled from two populations in Japan. Sixteen loci showed polymorphism in at least one population, with two to five alleles per locus. Observed and expected heterozygosities for the two populations ranged from 0.00 to 0.92 and 0.00 to 0.71, respectively.

conclusionsThe microsatellite markers developed here provide a useful tool to analyze clonal structure and sexual regeneration status and will help to manage the remaining genetic variation within C. japonicum.

Indexed as

clonal analysisconservation geneticsCypripedium japonicumIon PGM sequencingmicrosatellitesOrchidaceae

Identifiers

PMID26949576
PMCPMC4760748
OpenAlexW2297195549

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.