ArticleJournal of animal science and biotechnology2016
Sperm cryodamage occurs after rapid freezing phase: flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation.
Article in Journal of animal science and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- In vitro effects of green tea (Camellia sinensis) aqueous extract on sperm quality: Mitigating oxidative stress and sperm DNA fragmentation in freeze-dried human spermatozoa.Clinical and experimental reproductive medicine · 2026Article
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Raman spectroscopic analysis of intracellular ice-induced degradation of mesenchymal stromal cells.Biophysical journal · 2026Article
- Chitosan-coated nano-green-tea (Nano-GTE) in a tris-soybean lecithin extender improves post-thaw motility, antioxidant status, DNA integrity, and fertility of Ossimi Ram Semen.Scientific reports · 2026Article
- Cross-species multi-omic analyses of great ape ejaculates reveal novel strategies for enhancing sperm cryopreservation in Bornean orangutan (Pongo pygmaeus).Zoological letters · 2026Article
- Zinc oxide nanoparticles supplementation improve the quality and lipid profiles of frozen boar semen.Frontiers in veterinary science · 2026Article
- Article
- Proteomic Analysis of Frozen-Thawed Spermatozoa with Different Levels of Freezability in Dairy Goats.International journal of molecular sciences · 2023Article
- Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage.Frontiers in veterinary science · 2023Article
- The effect of different concentrations of taxifolin on the quality of frozen and thawed semen of Simmental cattle.Iranian journal of veterinary research · 2023Article
- Cryopreservation of Yak Semen: A Comprehensive Review.Animals : an open access journal from MDPI · 2022Review
- Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges.Journal of animal science and biotechnology · 2022Review
- First study ofVeterinary world · 2022Article
- Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment.Antioxidants (Basel, Switzerland) · 2022Review
- Article
- Association between Fatty Acid Composition, Cryotolerance and Fertility Competence of Progressively Motile Bovine Spermatozoa.Animals : an open access journal from MDPI · 2021Article
- Increased quality ofAnimal reproduction · 2020Article
- Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm.Veterinary medicine and science · 2020Article
- Changes in Bull Semen Metabolome in Relation to Cryopreservation and Fertility.Animals : an open access journal from MDPI · 2020Article
- Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability.Frontiers in veterinary science · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and antioxidant enzymes activity allows a more sensible evaluation of sperm cell during cryopreservation. The aim of this study was to evaluate sperm attributes and antioxidant enzymes activity during different stages of cryopreservation process. Semen samples from Holstein bulls (n = 4) were separated in 3 treatments: fresh (37 °C); cooled (5 °C); and thawed. Evaluation occurred at 0 h and 2 h after incubation. Membrane integrity, mitochondrial membrane potential (MMP) and DNA damages were evaluated by flow cytometry; activities of antioxidant enzymes such as catalase, superoxide dismutase and gluthatione peroxidase were measured by spectrofotometry.
resultsThere was an increase in the percentage of sperm with DNA damage in the thawed group, compared to fresh and cooled, and for 2 hs of incubation when compared to 0 h. Considering MMP, there was an increase in the percentage of cells with medium potential in thawed group when compared to fresh and cooled groups. Opposingly, a decrease was observed in the thawed group considering high mitochondrial potential. Also in the thawed group, there was an increase on cells with damaged acrosome and membrane when compared to fresh and cooled groups. Significant correlations were found between antioxidant enzymes activity and membrane or mitochondrial parameters.
conclusionBased on our results, we conclude that cryopreservation affects cellular and DNA integrity and that the critical moment is when sperm cells are exposed to freezing temperature. Also, our study indicates that intracellular antioxidant machinery (SOD and GPX enzymes) is not enough to control cryodamage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.