ArticleScientific reports2016
Investigation of the cause of geographic disparities in IDEXX ELISA sensitivity in serum samples from Mycobacterium bovis-infected cattle.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Anti-phenolic glycolipid antibodies inOne health (Amsterdam, Netherlands) · 2025Article
- Review on Bovine Tuberculosis: An Emerging Disease Associated with Multidrug-ResistantPathogens (Basel, Switzerland) · 2022Review
- Tuberculosis prevalence in animals and humans in the Republic of Kazakhstan.Veterinary world · 2021Article
- Study on supplemental test to improve the detection of bovine tuberculosis in individual animals and herds.BMC veterinary research · 2021Article
- Evaluation of the Efficacy of BCG in Protecting Against Contact Challenge With Bovine Tuberculosis in Holstein-Friesian and Zebu Crossbred Calves in Ethiopia.Frontiers in veterinary science · 2021Article
- Comparing recombinant MPB70/SahH and native 20-kDa protein for detecting bovine tuberculosis using ELISA.The Journal of veterinary medical science · 2020Article
- Review
- Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis.Clinical and vaccine immunology : CVI · 2017Article
- Early Detection of Circulating Antigen and IgM-Associated Immune Complexes during Experimental Mycobacterium bovis Infection in Cattle.Clinical and vaccine immunology : CVI · 2017Article
- Assessment of an ELISA method to support surveillance of bovine tuberculosis in Albania.Irish veterinary journal · 2015Article
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Authors and funding
9 authors.
Funding
Abstract
Accurately identifying Mycobacterium bovis-infected cattle is critical for bovine tuberculosis prevention and control. One method for identifying infected cattle is an ELISA developed by IDEXX laboratories, which detects antibodies to two M. bovis proteins, MPB70 and MPB83. The assay's sensitivity varies by geographic region, with sensitivities of 77%, 45%, and 9% in bovine serum samples from the United Kingdom (n = 126), the United States (n = 146), and Mexico (n = 128), respectively. We hypothesized that geographically-biased sequence variation in mpb70 and mpb83, or in the genes that regulate their expression (sigK and rskA), may explain these differing sensitivities. This hypothesis was tested by comparing the sequences of these four genes in 455 M. bovis strains isolated from cattle in the aforementioned countries. For each gene, a single, common sequence was identified in most genomes of the M. bovis strains collected in all three countries. Twelve of the 455 strains were isolated from infected cattle for which the IDEXX ELISA was also performed. Five of the seven ELISA-positive genomes and three of the five ELISA-negative genomes contained the most common sequence of all four genes. Thus, sequence variation in mpb70, mpb83, sigK, and rskA does not explain the geographic disparities in IDEXX ELISA sensitivity.
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