Evidence map›Paper›PMID 26948688›Full record

ArticleArchiv der Pharmazie2016

Synthesis of Arylazide- and Diazirine-Containing CrAsH-EDT2 Photoaffinity Probes.

Shameem S Syeda, Daren Rice, Derek J Hook, Leslie L Heckert, Gunda I Georg

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archiv der Pharmazie, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Shameem S SyedaDepartment of Medicinal Chemistry and Institute for Therapeutics Discovery and Development, University of Minnesota, Minneapolis, MN, USA.
Daren RiceInterdisciplinary Center for Male Contraceptive Research and Drug Development, University of Kansas Medical Center, Kansas City, KS, USA.
Derek J HookDepartment of Medicinal Chemistry and Institute for Therapeutics Discovery and Development, University of Minnesota, Minneapolis, MN, USA.
Leslie L HeckertInterdisciplinary Center for Male Contraceptive Research and Drug Development, University of Kansas Medical Center, Kansas City, KS, USA.
Gunda I GeorgDepartment of Medicinal Chemistry and Institute for Therapeutics Discovery and Development, University of Minnesota, Minneapolis, MN, USA.
University of Kansas Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two photo-crosslinking biarsenical (CrAsH-EDT2 )-modified probes were synthesized that are expected to be useful tools for tetracysteine-labeled proteins to facilitate the co-affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH-EDT2 and N(1) -(4-azido-2-nitrophenyl)hexane-1,6-diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3-related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest.

Indexed as

ArsenicArsenicalsAzidesCross-Linking ReagentsDiaminesDiazomethaneFluoresceinsHeLa CellsHumansMercaptoethanolPhotoaffinity LabelsStructure-Activity RelationshipTranscription Factors1,2-ethanedithiol4',5'-bis(1,2,3-dithioarsolan-2-yl)fluorescein-5-carboxylic acidArsenicArsenicalsAzidesCross-Linking ReagentsDiaminesDiazomethaneDMRT1 proteinFluoresceinsMercaptoethanolN(1)-(4-azido-2-nitrophenyl)hexane-1,6-diaminePhotoaffinity LabelsTranscription FactorsBiarsenical probeFluorescencePhotoaffinity probesTetracysteine-tagged recombinant DMRT1

Identifiers

PMID26948688
PMCPMC5069617
OpenAlexW2295696733

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.