ArticleScientific reports2016
Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Comprehensive identification and subcellular localization prediction of carbonic anhydrases in the marine haptophyte Tisochrysis lutea based on a refined genome annotation.Archives of microbiology · 2026Article
- IPTG- and estradiol-inducible gene expression systems in the unicellular red alga Cyanidioschyzon merolae.Plant physiology · 2026Article
- Characterization of Delta-7 Alkenone Desaturase in Haptophyte Gephyrocapsa huxleyi Through Heterologous Expression in Tisochrysis lutea.Marine biotechnology (New York, N.Y.) · 2025Article
- Exploring proteins within the coccolith matrix.Scientific reports · 2024Article
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects.Microorganisms · 2024Review
- Screening of antibiotics to obtain axenic cell cultures of a marine microalgaFrontiers in bioengineering and biotechnology · 2023Article
- Kre6 (yeast 1,6-β-transglycosylase) homolog, PhTGS, is essential for β-glucan synthesis in the haptophyteFrontiers in bioengineering and biotechnology · 2023Article
- Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae.Marine drugs · 2022Review
- Voltage-gated proton channels explain coccolithophore sensitivity to ocean acidification.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Association of Phosphatidylinositol-Specific Phospholipase C with Calcium-Induced Biomineralization in the CoccolithophoreMicroorganisms · 2020Article
- Genetic tool development in marine protists: emerging model organisms for experimental cell biology.Nature methods · 2020Article
- Simplified Transformation ofGenes · 2019Article
- Effect of KNOInternational journal of biological sciences · 2019Article
- Article
- Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory.World journal of microbiology & biotechnology · 2018Review
- Overexpression of Tisochrysis lutea Akd1 identifies a key cold-induced alkenone desaturase enzyme.Scientific reports · 2018Article
- Algal Cell Factories: Approaches, Applications, and Potentials.Marine drugs · 2016Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
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