ArticleScientific reports2016
Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Otic Drug Delivery Systems: Current and Future Perspectives.Current pharmaceutical design · 2026Review
- Peptides rapidly transport antibiotic across the intact tympanic membrane to treat a middle ear infection.Drug delivery · 2025Article
- Dynamic Nanopeptide Assemblies for Trans-Tympanic Drug Delivery.International journal of nanomedicine · 2025Article
- Peptide-Mediated Transport Across the Intact Tympanic Membrane Is Intracellular, with the Rate Determined by the Middle Ear Mucosal Epithelium.Biomolecules · 2024Article
- Ayurvedic Management of Presbycusis (Project TOPMAC): Protocol for an Exploratory Randomized Controlled Trial.JMIR research protocols · 2024Article
- Teprotumumab-associated chronic hearing loss screening and proposed treatments.BMJ case reports · 2022Article
- A transcytotic transport mechanism across the tympanic membrane.Scientific reports · 2022Article
- Drug Delivery across Barriers to the Middle and Inner Ear.Advanced functional materials · 2021Article
- Novel Antimicrobial Treatment Strategy Based on Drug Delivery Systems for Acute Otitis Media.Frontiers in pharmacology · 2021Review
- Niche- and Gender-Dependent Immune Reactions in Relation to the Microbiota Profile in Pediatric Patients with Otitis Media with Effusion.Infection and immunity · 2020Article
- Panel 1: Biotechnology, biomedical engineering and new models of otitis media.International journal of pediatric otorhinolaryngology · 2020Review
- Molecular Screening Strategy to Identify a Non-invasive Delivery Mechanism for the Treatment of Middle Ear Disorders.Frontiers in medicine · 2020Article
- Article
- Active Transport of Peptides Across the Intact Human Tympanic Membrane.Scientific reports · 2018Article
- Optimisation of peptides that actively cross the tympanic membrane by random amino acid extension: a phage display study.Journal of drug targeting · 2018Article
- Getting Drugs Across Biological Barriers.Advanced materials (Deerfield Beach, Fla.) · 2017Review
- Virus-Derived Peptides for Clinical Applications.Chemical reviews · 2017Review
- Peptides actively transported across the tympanic membrane: Functional and structural properties.PloS one · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Otitis media (OM) is a common pediatric disease for which systemic antibiotics are often prescribed. While local treatment would avoid the systemic treatment side-effects, the tympanic membrane (TM) represents an impenetrable barrier unless surgically breached. We hypothesized that the TM might harbor innate biological mechanisms that could mediate trans-TM transport. We used two M13-bacteriophage display biopanning strategies to search for mediators of trans-TM transport. First, aliquots of linear phage library displaying 10(10th) 12mer peptides were applied on the TM of rats with active bacterial OM. The middle ear (ME) contents were then harvested, amplified and the preparation re-applied for additional rounds. Second, the same naïve library was sequentially screened for phage exhibiting TM binding, internalization and then transit. Results revealed a novel set of peptides that transit across the TM to the ME in a time and temperature dependent manner. The peptides with highest transport capacities shared sequence similarities. Historically, the TM was viewed as an impermeable barrier. However, our studies reveal that it is possible to translocate peptide-linked small particles across the TM. This is the first comprehensive biopanning for the isolation of TM transiting peptidic ligands. The identified mechanism offers a new drug delivery platform into the ME.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.