ArticleStem cells (Dayton, Ohio)2016
Lineage-Specific Early Differentiation of Human Embryonic Stem Cells Requires a G2 Cell Cycle Pause.
Article in Stem cells (Dayton, Ohio), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Novel Genetic Determinants of Dental Maturation in Children.Journal of dental research · 2023Pooled it
- Embryonic Stem Cell-Specific Responses to DNA Replication Stress.bioRxiv : the preprint server for biology · 2025Article
- The specialized mitotic behavior of human embryonic stem cells.Cell and tissue research · 2022Article
- Imaging developmental cell cycles.Biophysical journal · 2021Review
- Lessons from the Embryo: an Unrejected Transplant and a Benign Tumor.Stem cell reviews and reports · 2021Review
- The Lineage Before Time: Circadian and Nonclassical Clock Influences on Development.Annual review of cell and developmental biology · 2020Review
- The cell cycle in stem cell proliferation, pluripotency and differentiation.Nature cell biology · 2019Review
- Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells.Scientific reports · 2018Article
- Magnesium Deprivation Potentiates Human Mesenchymal Stem Cell Transcriptional Remodeling.International journal of molecular sciences · 2018Article
- Wnt Signaling and Its Impact on Mitochondrial and Cell Cycle Dynamics in Pluripotent Stem Cells.Genes · 2018Review
- Cell cycle and pluripotency: Convergence on octamer‑binding transcription factor 4 (Review).Molecular medicine reports · 2017Review
- Unique Regulatory Mechanisms for the Human Embryonic Stem Cell Cycle.Journal of cellular physiology · 2017Review
- Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling.Stem cell reports · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Human embryonic stem cells (hESCs) have an abbreviated G1 phase of the cell cycle that allows rapid proliferation and maintenance of pluripotency. Lengthening of G1 corresponds to loss of pluripotency during differentiation. However, precise mechanisms that link alterations in the cell cycle and early differentiation remain to be defined. We investigated initial stages of mesendodermal lineage commitment in hESCs, and observed a cell cycle pause. Transcriptome profiling identified several genes with known roles in regulation of the G2/M transition that were differentially expressed early during lineage commitment. WEE1 kinase, which blocks entry into mitosis by phosphorylating CDK1 at Y15, was the most highly expressed of these genes. Inhibition of CDK1 phosphorylation by a specific inhibitor of WEE1 restored cell cycle progression by preventing the G2 pause. Directed differentiation of hESCs revealed that cells paused during commitment to the endo- and mesodermal, but not ectodermal, lineages. Functionally, WEE1 inhibition during meso- and endodermal differentiation selectively decreased expression of definitive endodermal markers SOX17 and FOXA2. Our findings identify a novel G2 cell cycle pause that is required for endodermal differentiation and provide important new mechanistic insights into early events of lineage commitment. Stem Cells 2016;34:1765-1775.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.