ArticleCytotechnology2016
The effect of a number of H9C2 rat cardiomyocytes passage on repeatability of cytotoxicity study results.
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evaluation of the cardioprotective potential of extracellular vesicles - a systematic review and meta-analysis.Scientific reports · 2018Pooled it
- Linking Experimental Models to Pathophysiology: Oxidative Stress and DNA Damage in Cardiovascular Diseases.International journal of molecular sciences · 2026Review
- Combined effects of gabapentin with verapamil and metoprolol on cardiovascular function of anesthetized male rats.European journal of pharmacology · 2025Article
- Imatinib‑ and ponatinib‑mediated cardiotoxicity in zebrafish embryos and H9c2 cardiomyoblasts.Molecular medicine reports · 2024Article
- Vaccarin Ameliorates Doxorubicin-Induced Cardiotoxicity via Inhibition of p38 MAPK Mediated Mitochondrial Dysfunction.Journal of cardiovascular translational research · 2024Article
- Tailoring transfection for cardiomyocyte cell lines: balancing efficiency and toxicity in lipid versus polymer-based transfection methods in H9c2 and HL-1 cells.American journal of physiology. Heart and circulatory physiology · 2024Article
- Therapeutic Potential ofCardiovascular & hematological agents in medicinal chemistry · 2024Article
- Ultrastructural changes in cardiac and skeletal myoblasts following in vitro exposure to monensin, salinomycin, and lasalocid.PloS one · 2024Article
- Optimization of H9c2 differentiation leads to calcium-active and striated cardiac cells without addition of retinoic acid.Frontiers in cell and developmental biology · 2024Article
- Cardioprotective Role ofCardiovascular & hematological agents in medicinal chemistry · 2024Article
- Cardioprotective Effect ofMolecules (Basel, Switzerland) · 2023Article
- In Vitro Toxicological Profile of Labetalol-Folic Acid/Folate Co-Administration in H9c2(2-1) and HepaRG Cells.Medicina (Kaunas, Lithuania) · 2022Article
- Yttrium chloride-induced cytotoxicity and DNA damage response via ROS generation and inhibition of Nrf2/PPARγ pathways in H9c2 cardiomyocytes.Archives of toxicology · 2022Article
- Metabolites of Cannabis Induce Cardiac Toxicity and Morphological Alterations in Cardiac Myocytes.International journal of molecular sciences · 2022Article
- Inhibition of mitochondrial respiration has fundamentally different effects on proliferation, cell survival and stress response in immature versus differentiated cardiomyocyte cell lines.Frontiers in cell and developmental biology · 2022Article
- Rutin bioconjugates as potential nutraceutical prodrugs: AnFrontiers in pharmacology · 2022Article
- Extracellular vesicles from thalassemia patients carry iron-containing ferritin and hemichrome that promote cardiac cell proliferation.Annals of hematology · 2021Article
- Expression of cell adhesion molecule, Gicerin/CD146 during the formation of heart and in the cardiac hypertrophy.Molecular and cellular biochemistry · 2021Article
- Cocaine Induces Cytoskeletal Changes in Cardiac Myocytes: Implications for Cardiac Morphology.International journal of molecular sciences · 2021Article
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7 authors.
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Abstract
The embryonic cardiomyocyte cell line H9C2 is commonly used in numerous in vitro studies, including cardiotoxicity analyses of new drugs. So far no results were published for studies on cell parameters variability during the cell line ageing process. For this reason the aim of the study was to evaluate the effect of a number of H9C2 rat embryonic cardiomyocytes passages on repeatability of study results for selected cytotoxicity parameters, with doxorubicin as a model toxic agent. The cultures were passaged twenty-five times. Cells from passage 1, 5, 10, 15, 20 and 25 were treated with doxorubicin for 24 h. Then drug cytotoxicity was evaluated with the MTT test and additionally the nuclear factor erythroid 2-related factor (Nrf2) gene expression was examined. The analysis of oxidative stress intensity and cell morphology was also assessed. The microscopic appearance of cells indicates that untreated cardiomyocytes morphology changes as well as sensitivity to toxic effects increases with the number of passages. Also an increase in oxidative stress in cells occurs with further passaging of cardiomyocytes. Statistical significance of differences in conducted tests results depended on doxorubicin concentration but in many cases the H9C2 line was found to be a reliable in vitro model only for the first five passages. For this reason it is important to take into consideration that further culturing of cardiomyocytes may not ensure repeatability of study results due to the culture ageing.
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