ArticleTalanta2016
Derivatization of estrogens enhances specificity and sensitivity of analysis of human plasma and serum by liquid chromatography tandem mass spectrometry.
Article in Talanta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systemic estradiol levels with low-dose vaginal estrogens.Menopause (New York, N.Y.) · 2020Pooled it
- Aromatase Inhibition Reduces Insulin Sensitivity in Healthy Men.The Journal of clinical endocrinology and metabolism · 2016Trial
- Systematic Optimization of an SLE-DAPTAD LC-MS/MS Workflow for Simultaneous Quantification of Low-Abundance Vitamin DRapid communications in mass spectrometry : RCM · 2026Article
- Targeted Livestock Metabolomics: A Review of Liquid chromatography-Mass Spectrometry-Based Approaches.Journal of agricultural and food chemistry · 2026Review
- Differentiation of steroid isomers via derivatization and electron-activated dissociation tandem mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- Ovarian Escape in Hormone Receptor-Positive Breast Cancer: Mechanisms, Risk Factors, Clinical Consequences, and Management Strategies.Breast cancer (Dove Medical Press) · 2026Review
- Cardiac PAD2 expression and myocardial citrullination decline with age in female mice independent of estrogen.American journal of physiology. Heart and circulatory physiology · 2025Article
- Estrogen profiling in blood and brain: Effects of season and an aggressive interaction in a songbird.PloS one · 2025Article
- Mimicking women's endocrine milieu in mice for women's health-related studies.npj women's health · 2025Article
- Rapid Analysis of Estrogens in Meat Samples by High Performance Liquid Chromatography with Fluorescence Detection.Journal of fluorescence · 2024Article
- Derivatization procedure of estradiol with a combination of MPDNP-F and 4-dimethylaminopyridine to generate product ion containing estradiol-skeleton for reliable determination of its serum/plasma concentrations by LC/ESI-MS/MS.Analytical and bioanalytical chemistry · 2024Article
- Comparing derivatization reagents for quantitative LC-MS/MS analysis of a variety of vitamin D metabolites.Analytical and bioanalytical chemistry · 2023Article
- Stability of sample extracts of vitamin DAnalytical and bioanalytical chemistry · 2023Article
- Ruxolitinib and exemestane for estrogen receptor positive, aromatase inhibitor resistant advanced breast cancer.NPJ breast cancer · 2022Article
- Associations of established breast cancer risk factors with urinary estrogens in postmenopausal women.Cancer causes & control : CCC · 2022Article
- Effects of systemic inflammation on relapse in early breast cancer.NPJ breast cancer · 2021Article
- Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.Journal of pharmaceutical and biomedical analysis · 2020Article
- LC-HRMS of derivatized urinary estrogens and estrogen metabolites in postmenopausal women.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2020Article
- Simultaneous quantification of estrogens and glucocorticoids in human adipose tissue by liquid-chromatography-tandem mass spectrometry.The Journal of steroid biochemistry and molecular biology · 2019Article
- Current strategies for quantification of estrogens in clinical research.The Journal of steroid biochemistry and molecular biology · 2019Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Estrogens circulate at concentrations less than 20pg/mL in men and postmenopausal women, presenting analytical challenges. Quantitation by immunoassay is unreliable at these low concentrations. Liquid chromatography tandem mass spectrometry (LC-MS/MS) offers greater specificity and sometimes greater sensitivity, but ionization of estrogens is inefficient. Introduction of charged moieties may enhance ionization, but many such derivatives of estrogens generate non-specific product ions originating from the "reagent" group. Therefore an approach generating derivatives with product ions specific to individual estrogens was sought. Estrogens were extracted from human plasma and serum using solid phase extraction and derivatized using 2-fluoro-1-methylpyridinium-p-toluenesulfonate (FMP-TS). Electrospray in positive mode with multiple reaction monitoring using a QTrap 5500 mass spectrometer was used to quantify "FMP" derivatives of estrogens, following LC separation. Transitions for the FMP derivatives of estrone (E1) and estradiol (E2) were compound specific (m/z 362→238 and m/z 364→128, respectively). The limits of detection and quantitation were 0.2pg on-column and the method was linear from 1-400pg/sample. Measures of intra- and inter-assay variability, precision and accuracy were acceptable (<20%). The derivatives were stable over 24h at 10°C (7-9% degradation). Using this approach, E1 and E2, respectively were detected in human plasma and serum: pre-menopausal female serum (0.5mL) 135-473, 193-722pmol/L; male plasma (1mL) 25-111, 60-180pmol/L and post-menopausal female plasma (2mL), 22-78, 29-50pmol/L. Thus FMP derivatization, in conjunction with LC-MS/MS, is suitable for quantitative analysis of estrogens in low abundance in plasma and serum, offering advantages in specificity over immunoassay and existing MS techniques.
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