ArticlePlanta2016
The Antirrhinum AmDEL gene enhances flavonoids accumulation and salt and drought tolerance in transgenic Arabidopsis.
Article in Planta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 76 citations in OpenAlex.
- Genome-Wide Identification and Characterization of bHLH Family inLife (Basel, Switzerland) · 2026Article
- Insights Into the Regulation of Salt Tolerance by Flavonoid and Cyanoamino Acid Metabolism in Sorghum-Sudangrass Hybrids.Plant direct · 2026Article
- Flavonoids in Plant Salt Stress Responses: Biosynthesis, Regulation, Functions, and Signaling Networks.Plants (Basel, Switzerland) · 2026Review
- Integrated multi-omics analysis uncovers key metabolic and transcriptional regulatory networks inFrontiers in plant science · 2026Article
- Co-Analysis of Transcriptome and Metabolome Reveals Flavonoid Biosynthesis inFoods (Basel, Switzerland) · 2025Article
- Ectopic Expression of a Poplar GenePlants (Basel, Switzerland) · 2025Article
- Abiotic stress-induced changes in Tetrastigma hemsleyanum: insights from secondary metabolite biosynthesis and enhancement of plant defense mechanisms.BMC plant biology · 2024Article
- Heterologous Expression of MYB Gene (International journal of molecular sciences · 2024Article
- Integrated Analysis of Transcriptome and Metabolome Provides Insights into Flavonoid Biosynthesis of Blueberry Leaves in Response to Drought Stress.International journal of molecular sciences · 2024Article
- Multi-omics analysis reveals key regulatory defense pathways and genes involved in salt tolerance of rose plants.Horticulture research · 2024Article
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- Sensitivity of the Photosynthetic Apparatus in Maize and Sorghum under Different Drought Levels.Plants (Basel, Switzerland) · 2023Article
- A Novel bHLH Transcription Factor PtrbHLH66 from Trifoliate Orange Positively Regulates Plant Drought Tolerance by Mediating Root Growth and ROS Scavenging.International journal of molecular sciences · 2022Article
- Diverse Physiological Roles of Flavonoids in Plant Environmental Stress Responses and Tolerance.Plants (Basel, Switzerland) · 2022Review
- Overexpression ofInternational journal of molecular sciences · 2022Article
- Purple stemFrontiers in plant science · 2022Article
- Systematic Analysis of bHLH Transcription Factors in Cassava Uncovers Their Roles in Postharvest Physiological Deterioration and Cyanogenic Glycosides Biosynthesis.Frontiers in plant science · 2022Article
- Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance.International journal of molecular sciences · 2021Review
- Combined Transcriptomic and Metabolomic Analysis Reveals the Role of Phenylpropanoid Biosynthesis Pathway in the Salt Tolerance Process ofInternational journal of molecular sciences · 2021Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionA basic helix-loop-helix (bHLH) transcription factor gene from Antirrhinum, AmDEL , increases flavonoids accumulation and enhances salt and drought tolerance via up-regulating flavonoid biosynthesis, proline biosynthesis and ROS scavenging genes in transgenic Arabidopsis. In plants, transcriptional regulation is the most important tools for increasing flavonoid biosynthesis. The AmDEL gene, as a basic helix-loop-helix transcription factor gene from Antirrhinum, has been shown to increase flavonoids accumulation in tomato. However, its role in tolerance to abiotic stresses has not yet been investigated. In this study, the codon-optimized AmDEL gene was chemically synthesized. Subcellular localization analysis in onion epidermal cells indicated that AmDEL protein was localized to the nucleus. Expression analysis in yeast showed that the full length of AmDEL exhibited transcriptional activation. Overexpression of AmDEL significantly increased flavonoids accumulation and enhanced salt and drought tolerance in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that overexpression of AmDEL resulted in the up-regulation of genes involved in flavonoid biosynthesis, proline biosynthesis and ROS scavenging under salt and drought stresses. Meanwhile, Western blot and enzymatic analyses showed that the activities of phenylalanine ammonia lyase, chalcone isomerase, dihydroflavonol reductase, pyrroline-5-carboxylate synthase, superoxide dismutase and peroxidase were also increased. Further components analyses indicated that the significant increase of proline and relative water content and the significant reduction of H2O2 and malonaldehyde content were observed under salt and drought stresses. In addition, the rates of electrolyte leakage and water loss were reduced in transgenic plants. These findings imply functions of AmDEL in accumulation of flavonoids and tolerance to salt and drought stresses. The AmDEL gene has the potential to be used to increase the content of valuable flavonoids and improve tolerance to abiotic stresses in plants.
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