ArticleActa biomaterialia2016
Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels.
Article in Acta biomaterialia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 107 citations in OpenAlex.
- An overview of recent flexible- and soft-biomaterial applications in myocardial infarction and other cardiovascular diseases.Materials today. Bio · 2026Review
- Anti-Inflammatory and Angiogenic Effects of Stem Cell Secretome.International journal of molecular sciences · 2026Article
- Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support.Bioactive materials · 2025Article
- Clickable PEG-norbornene microgels support suspension bioprinting and microvascular assembly.Acta biomaterialia · 2025Article
- Biofabrication and Characterization of Vascularizing PEG-Norbornene Microgels.Journal of biomedical materials research. Part A · 2025Article
- Semaphorin-4D signaling in recruiting dental stem cells for vascular stabilization.Stem cell research & therapy · 2025Article
- Importance of Choriocapillaris Replacement in Therapeutic Strategies for Age-Related Macular Degeneration.Advances in experimental medicine and biology · 2025Review
- Technology for the formation of engineered microvascular network models and their biomedical applications.Nano convergence · 2024Review
- Review: Human stem cell-based 3D in vitro angiogenesis models for preclinical drug screening applications.Molecular biology reports · 2024Review
- Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells.APL bioengineering · 2023Article
- The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research.International journal of molecular sciences · 2023Review
- A combination of matrix stiffness and degradability dictate microvascular network assembly and remodeling in cell-laden poly(ethylene glycol) hydrogels.Biomaterials · 2023Article
- Engineering approaches for cardiac organoid formation and their characterization.Translational research : the journal of laboratory and clinical medicine · 2022Review
- Catalyst-Free Click Chemistry for Engineering Chondroitin Sulfate-Multiarmed PEG Hydrogels for Skin Tissue Engineering.Journal of functional biomaterials · 2022Article
- Review
- Tissue Engineered Neurovascularization Strategies for Craniofacial Tissue Regeneration.ACS applied bio materials · 2022Review
- A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?Frontiers in bioengineering and biotechnology · 2022Review
- 3D hydrogel models of the neurovascular unit to investigate blood-brain barrier dysfunction.Neuronal signaling · 2021Review
- Cardiac Tissue Engineering for the Treatment of Myocardial Infarction.Journal of cardiovascular development and disease · 2021Review
- Design considerations for engineering 3D models to study vascular pathologies in vitro.Acta biomaterialia · 2021Review
Corrections and comments
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Authors and funding
16 authors at 2 institutions in 1 country.
Funding
Abstract
Here, we describe an in vitro strategy to model vascular morphogenesis where human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) are encapsulated in peptide-functionalized poly(ethylene glycol) (PEG) hydrogels, either on standard well plates or within a passive pumping polydimethylsiloxane (PDMS) tri-channel microfluidic device. PEG hydrogels permissive towards cellular remodeling were fabricated using thiol-ene photopolymerization to incorporate matrix metalloproteinase (MMP)-degradable crosslinks and CRGDS cell adhesion peptide. Time lapse microscopy, immunofluorescence imaging, and RNA sequencing (RNA-Seq) demonstrated that iPSC-ECs formed vascular networks through mechanisms that were consistent with in vivo vasculogenesis and angiogenesis when cultured in PEG hydrogels. Migrating iPSC-ECs condensed into clusters, elongated into tubules, and formed polygonal networks through sprouting. Genes upregulated for iPSC-ECs cultured in PEG hydrogels relative to control cells on tissue culture polystyrene (TCP) surfaces included adhesion, matrix remodeling, and Notch signaling pathway genes relevant to in vivo vascular development. Vascular networks with lumens were stable for at least 14days when iPSC-ECs were encapsulated in PEG hydrogels that were polymerized within the central channel of the microfluidic device. Therefore, iPSC-ECs cultured in peptide-functionalized PEG hydrogels offer a defined platform for investigating vascular morphogenesis in vitro using both standard and microfluidic formats. STATEMENT OF SIGNIFICANCE: Human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) cultured in synthetic hydrogels self-assemble into capillary networks through mechanisms consistent with in vivo vascular morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.