ArticleThe Journal of biological chemistry2016
The Sodium Glucose Cotransporter SGLT1 Is an Extremely Efficient Facilitator of Passive Water Transport.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Yeast Complementation Assays Demonstrating the Importance of the Affinity Tag Position in Membrane Protein Purification, as Exemplified bySmall science · 2025Article
- SWEET family transporters act as water conducting carrier proteins in plants.bioRxiv : the preprint server for biology · 2024Article
- Photolipid excitation triggers depolarizing optocapacitive currents and action potentials.Nature communications · 2024Article
- Photolipid excitation triggers depolarizing optocapacitive currents and action potentials.bioRxiv : the preprint server for biology · 2023Article
- Influence of SGLT1 Sugar Uptake Inhibitors on Water Transport.Molecules (Basel, Switzerland) · 2023Article
- Entropic barrier of water permeation through single-file channels.Communications chemistry · 2023Article
- Collective Domain Motion Facilitates Water Transport in SGLT1.International journal of molecular sciences · 2023Article
- Honey, I shrunk the extracellular space: Measurements and mechanisms of astrocyte swelling.Glia · 2022Review
- Modeling of SGLT1 in Reconstituted Systems Reveals Apparent Ion-Dependencies of Glucose Uptake and Strengthens the Notion of Water-Permeable Apo States.Frontiers in physiology · 2022Article
- ScatteringNanoscale advances · 2021Article
- Native-like membrane models of E. coli polar lipid extract shed light on the importance of lipid composition complexity.BMC biology · 2021Article
- A comprehensive overview of substrate specificity of glycoside hydrolases and transporters in the small intestine : "A gut feeling".Cellular and molecular life sciences : CMLS · 2020Review
- Glucose transporters in the small intestine in health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- Pathophysiology of SARS-CoV-2 in Lung of Diabetic Patients.Frontiers in physiology · 2020Review
- PRDM9 forms a trimer by interactions within the zinc finger array.Life science alliance · 2019Article
- Positively charged residues at the channel mouth boost single-file water flow.Faraday discussions · 2018Article
- Single-file transport of water through membrane channels.Faraday discussions · 2018Article
- Molecular Dynamics Simulation for All.Neuron · 2018Review
- Quantification of Water Flux in Vesicular Systems.Scientific reports · 2018Article
- Chloride Dysregulation, Seizures, and Cerebral Edema: A Relationship with Therapeutic Potential.Trends in neurosciences · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The small intestine is void of aquaporins adept at facilitating vectorial water transport, and yet it reabsorbs ∼8 liters of fluid daily. Implications of the sodium glucose cotransporter SGLT1 in either pumping water or passively channeling water contrast with its reported water transporting capacity, which lags behind that of aquaporin-1 by 3 orders of magnitude. Here we overexpressed SGLT1 in MDCK cell monolayers and reconstituted the purified transporter into proteoliposomes. We observed the rate of osmotic proteoliposome deflation by light scattering. Fluorescence correlation spectroscopy served to assess (i) SGLT1 abundance in both vesicles and plasma membranes and (ii) flow-mediated dilution of an aqueous dye adjacent to the cell monolayer. Calculation of the unitary water channel permeability, pf, yielded similar values for cell and proteoliposome experiments. Neither the absence of glucose or Na(+), nor the lack of membrane voltage in vesicles, nor the directionality of water flow grossly altered pf Such weak dependence on protein conformation indicates that a water-impermeable occluded state (glucose and Na(+) in their binding pockets) lasts for only a minor fraction of the transport cycle or, alternatively, that occlusion of the substrate does not render the transporter water-impermeable as was suggested by computational studies of the bacterial homologue vSGLT. Although the similarity between the pf values of SGLT1 and aquaporin-1 makes a transcellular pathway plausible, it renders water pumping physiologically negligible because the passive flux would be orders of magnitude larger.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.