ArticleG3 (Bethesda, Md.)2016
Mapping Challenging Mutations by Whole-Genome Sequencing.
Article in G3 (Bethesda, Md.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Characterization ofbioRxiv : the preprint server for biology · 2026Article
- Integrin is required for basement membrane crossing and branching of an invading intracellular tube.Development (Cambridge, England) · 2026Article
- Whole-genome sequencing reveals novel ryanodine receptor mutations P4568L and Y4701D conferring high-level chlorantraniliprole resistance in Spodoptera litura (Lepidoptera: Noctuidae).Pest management science · 2026Article
- The EGF-motif-containing protein SPE-36 is a secreted sperm protein required for fertilization in C. elegans.Current biology : CB · 2023Article
- CRISPR-mediated genome editing allows for efficient on demand creation of >200 kb deficiencies with precise boundaries.microPublication biology · 2023Article
- A combinatorial strategy to identify various types of QTLs for quantitative traits using extreme phenotype individuals in an FPlant communications · 2022Article
- Article
- Mutation Mapping and Identification by Whole-Genome Sequencing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.G3 (Bethesda, Md.) · 2021Article
- Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans.Scientific reports · 2021Article
- The molecular underpinnings of fertility: Genetic approaches inAdvanced genetics (Hoboken, N.J.) · 2021Article
- Harnessing the power of genetics: fast forward genetics in Caenorhabditis elegans.Molecular genetics and genomics : MGG · 2021Review
- Next-generation forward genetic screens: using simulated data to improve the design of mapping-by-sequencing experiments in Arabidopsis.Nucleic acids research · 2019Article
- The zinc transporter ZIPT-7.1 regulates sperm activation in nematodes.PLoS biology · 2018Article
- Use of a Sibling Subtraction Method for Identifying Causal Mutations inG3 (Bethesda, Md.) · 2018Article
- Article
- MIP-MAP: High-Throughput Mapping ofGenetics · 2017Article
- Evaluating alignment and variant-calling software for mutation identification in C. elegans by whole-genome sequencing.PloS one · 2017Article
- Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans.Genetics · 2016Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Whole-genome sequencing provides a rapid and powerful method for identifying mutations on a global scale, and has spurred a renewed enthusiasm for classical genetic screens in model organisms. The most commonly characterized category of mutation consists of monogenic, recessive traits, due to their genetic tractability. Therefore, most of the mapping methods for mutation identification by whole-genome sequencing are directed toward alleles that fulfill those criteria (i.e., single-gene, homozygous variants). However, such approaches are not entirely suitable for the characterization of a variety of more challenging mutations, such as dominant and semidominant alleles or multigenic traits. Therefore, we have developed strategies for the identification of those classes of mutations, using polymorphism mapping in Caenorhabditis elegans as our model for validation. We also report an alternative approach for mutation identification from traditional recombinant crosses, and a solution to the technical challenge of sequencing sterile or terminally arrested strains where population size is limiting. The methods described herein extend the applicability of whole-genome sequencing to a broader spectrum of mutations, including classes that are difficult to map by traditional means.
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Registered trials
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