ArticleJournal of nanobiotechnology2016
An in vitro alveolar macrophage assay for predicting the short-term inhalation toxicity of nanomaterials.
Article in Journal of nanobiotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages.International journal of molecular sciences · 2020Pooled it
- Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening.Stem cell research & therapy · 2026Article
- Macrophages bend long fibres with flexural rigidity lower than 3 mN·nmParticle and fibre toxicology · 2026Article
- Proteomic Profiling of Alveolar Macrophages Identifies Loss of Lysosomal Content as an Indicator of Nanofiber-Induced Frustrated Phagocytosis.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Readiness of theFrontiers in toxicology · 2026Article
- Application of in vitro pulmonary models for hazard screening of silica particles.Archives of toxicology · 2025Article
- Towards a harmonized testing strategy for nanofibers by integrating toxicological screening and proteomic profiling.Scientific reports · 2025Article
- New approach methodologies for in vitro toxicity screening of nanomaterial using a pulmonary three-dimensional floating extracellular matrix model.Journal of biological engineering · 2025Article
- Occupationally Relevant Zinc- and Copper-Containing Metal Fumes Inhibit Human THP-1 Macrophage TNF and IL-6 Responses to Bacterial Stimuli.Global challenges (Hoboken, NJ) · 2025Article
- Risk characterization for silica-related silicosis and lung cancer in communities adjacent to sand and gravel extraction facilities: examining limitations in our current risk methods.Frontiers in public health · 2025Article
- Methodological steps forward in toxicological in vitro screening of mineral wools in primary rat alveolar macrophages and normal rat mesothelial NRM2 cells.Archives of toxicology · 2024Article
- Towards characterization of cell culture conditions for reliable proteomic analysis: in vitro studies on A549, differentiated THP-1, and NR8383 cell lines.Archives of toxicology · 2024Article
- Article
- Differences in the anatomy and physiology of the human and rat respiratory tracts and impact on toxicological assessments.Regulatory toxicology and pharmacology : RTP · 2024Review
- Synthetic silica fibers of different length, diameter and shape: synthesis and interaction with rat (NR8383) and human (THP-1) macrophages in vitro, including chemotaxis and gene expression profile.Particle and fibre toxicology · 2024Article
- Evaluation of the immunotoxicity potential of nanomaterials using THP-1 cells.Frontiers in toxicology · 2024Article
- Cell-Biological Response and Sub-Toxic Inflammatory Effects of Titanium Dioxide Particles with Defined Polymorphic Phase, Size, and Shape.Nanomaterials (Basel, Switzerland) · 2023Article
- Metal-based nanomaterials and nanocomposites as promising frontier in cancer chemotherapy.MedComm · 2023Review
- Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users.F1000Research · 2023Review
- Predicting nanomaterials pulmonary toxicity in animals by cell culture models: Achievements and perspectives.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
2 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
backgroundMost in vitro studies investigating nanomaterial pulmonary toxicity poorly correlate to in vivo inhalation studies. Alveolar macrophages (AMs) play an outstanding role during inhalation exposure since they effectively clear the alveoli from particles. This study addresses the applicability of an in vitro alveolar macrophage assay to distinguish biologically active from passive nanomaterials.
methodsRat NR8383 alveolar macrophages were exposed to 18 inorganic nanomaterials, covering AlOOH, BaSO4, CeO2, Fe2O3, TiO2, ZrO2, and ZnO NMs, amorphous SiO2 and graphite nanoplatelets, and two nanosized organic pigments. ZrO2 and amorphous SiO2 were tested without and with surface functionalization. Non-nanosized quartz DQ12 and corundum were used as positive and negative controls, respectively. The test materials were incubated with the cells in protein-free culture medium. Lactate dehydrogenase, glucuronidase, and tumour necrosis factor alpha were assessed after 16 h. In parallel, H2O2 was assessed after 1.5 h. Using the no-observed-adverse-effect concentrations (NOAECs) from available rat short-term inhalation studies (STIS), the test materials were categorized as active (NOAEC < 10 mg/m(3)) or passive.
resultsIn vitro data reflected the STIS categorization if a particle surface area-based threshold of <6000 mm(2)/mL was used to determine the biological relevance of the lowest observed significant in vitro effects. Significant effects that were recorded above this threshold were assessed as resulting from test material-unspecific cellular 'overload'. Test materials were assessed as active if ≥2 of the 4 in vitro parameters undercut this threshold. They were assessed as passive if 0 or 1 parameter was altered. An overall assay accuracy of 95 % was achieved.
conclusionsThe in vitro NR8383 alveolar macrophage assay allows distinguishing active from passive nanomaterials. Thereby, it allows determining whether in vivo short-term inhalation testing is necessary for hazard assessment. Results may also be used to group nanomaterials by biological activity. Further work should aim at validating the assay.
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